摘要
目的:探讨α-细辛脑脂质聚合物杂化纳米粒(ARE-LPHNPs)的工艺优化及表征。方法:采用自组装法制备α-细辛脑脂质聚合物杂化纳米粒,单因素试验考察卵磷脂与mPEG-PLGA的比例、mPEG-PLGA质量、有机相与水相体积比及药载比的影响,星点设计-效应面法优化处方工艺,并对其理化性质进行表征。结果:ARE-LPHNPs最优处方为mPEG-PLGA质量为4.69mg、有机相与水相体积比1∶2.08,药载比为19.74%。最优工艺制备出的ARE-LPHNPs外观呈球形,平均粒径及电位分别为(87.67±1.25)nm,(-18.1±0.99)mV,包封率与载药量分别为(84.17±0.37)%、(7.63±0.05)%。DSC结果表明药物在ARE-LPHNPs中未以结晶形式存在。结论:采用自组装法制备ARE-LPHNPs,工艺稳定,所制备纳米粒外观圆整,可以为进一步体内研究提供参考。
Objective：To optimize the formulation of the lipid polymer hybrid nanoparticles loaded withα-Asarone,and characterize its physical and chemical properties.Methods ：ARE-LPHNPs was prepared by using self-assembly method,four factors including the ratio of lecithin to mPEG-PLGA,the mass of the mPEG-PLGA,volume ratio of organic phase to aqueous phase and the ratio of drug to mPEG-PLGA were investigated,then to optimize the preparation of ARE-LPHNPs by the central composite design response surface method,and to study its physicochemical properties.Result：The optimal process conditions of ARE-LPHNPs were as follows,the mass of the mPEG-PLGA was 4.69 mg,volume ratio of organic phase to aqueous phase was 1：2.08 and the ratio of drug to mPEG-PLGA was 19.74%.According to the optimal conditions,the morphological of ARE-LPHNPs was spherical in shape,the mean diameter,entrapment efficiency,drug loading were（87.67±1.25）nm,（84.17±0.37）%、（7.63±0.05）%,respectively.Conclusion：ARE-LPHNPs was successfully prepared by self assembly method,the process is stable and easy to operate,the morphology of nanoparticles is spherical,and provide reference for the further development for the in vivo study.
作者
蒋楠
穆珺
王春柳
张红
刘洋
马虎强
李晔
Jiang Nan;Mu Jun;Wang Chunliu(Luoyang Central Hospital Affilated to Zhengzhou University（ Luoyang 471000)
出处
《陕西中医》
2018年第6期800-805,共6页
Shaanxi Journal of Traditional Chinese Medicine
基金
西安市科技计划项目[(2017122SF/YX016(7)]
关键词
石菖蒲
中药开发
@α-细辛脑脂质聚合物
Shichangpu Development of Chinese materia meclica @a-Asarum brain lipid polymer
