摘要
目的：观察α-半乳糖神经酰胺（α-GalCer）对哮喘小鼠肺树突状细胞（LDCs）表面分子和分泌细胞因子水平的影响。方法：将24只BALB/c小鼠随机分为正常对照组、哮喘组和哮喘α-GalCer组,每组8只。以卵清白蛋白（OVA）诱导哮喘动物模型,分别以α-GalCer或PBS干预。流式细胞术检测小鼠LDCs表面分子MHC-Ⅱ、CD40、CD86和CD80的表达水平,ELISA法检测小鼠LDCs培养上清液IL-12p70、IL-6、TNF-α和IL-10的水平;同时检测小鼠肺iNKT细胞数量和分泌细胞因子水平。结果：与哮喘组比较,哮喘α-GalCer组小鼠LDCs表面分子MHC-Ⅱ、CD40、CD86和CD80表达水平明显升高（P〈0.01）,LDCs体外培养上清液IL-12p70、IL-6和TNF-α水平明显升高P〈0.01或P〈0.05）;同时哮喘α-GalCer组小鼠肺iNKT细胞数量和分泌细胞因子水平明显增高（P〈0.01或P〈0.05）。结论：α-GalCer可以增强哮喘小鼠LDCs表面分子和促炎细胞因子表达水平,这可能与其活化肺iNKT细胞有关。
Objective：To investigate the effects ofα-GalCer on the surface maturation markers expression and cytokine secretion of lung dendritic cells（LDCs）in a mouse model of asthma.Methods：Twenty-four wild-type BALB/c mice were randomly divided into control group,asthma group（OVA-induced asthmatic mice treated with PBS）,and asthmaα-GalCer group（OVA-induced asthmatic mice treated withα-GalCer）.Flow cytometric analysis was used to detect the expression of MHC-Ⅱ,CD40,CD86,and CD80 of LDCs and the number of lung iNKT cells,IL-4＋and IFN-γ＋iNKT cells.Levels of proinflammatory cytokine in the culture supernatant of LDCs were measured by ELISA.Results：The expression of MHC-Ⅱ,CD40,CD86,and CD80 of LDCs fromα-GalCer group was markedly elevated,compared with those in asthma group（P〈0.01 or P〈0.05）.The concentrations of IL-12 p70,IL-6,and TNF-αwere higher in the culture supernatant of LDCs inα-GalCer group than in asthma group（P〈0.01 or P〈0.05）.Meanwhile,the number and activity of lung iNKT cells was significantly higher inα-GalCer group than those inasthma group（P〈0.01）.Conclusion：α-GalCer can enhance the expression of surface maturation markers and secretion of proinflammatory cytokine of LDCs in a mouse asthma model,which maybe involve in activation of lung iNKT cells.
作者
郭旭雪
聂汉祥
陈千慧
陈硕
邓霓姗
GUO Xuxue;NIE Hanxiang;CHEN Qianhui;CHEN Shuo;DENG Nishan(Dept. of Respiratory Medicine, Renmin Hospital of Wuhan University, Wuhan 430060, Chin)
出处
《武汉大学学报（医学版）》
CAS
2018年第4期565-569,共5页
Medical Journal of Wuhan University
基金
国家自然科学基金资助项目(编号:81770036)
