摘要
24-乙酰泽泻醇A作为泽泻的主要组成部分有抗动脉粥样硬化（atherosclerosis,AS）作用且AS与血管平滑肌细胞（vascular smooth muscle cells,VSMCs）增殖密切相关,故该研究通过活体取材及培养大鼠腹主动脉VSMCs,50 mg·L-1ox-LDL诱导VSMCs建立细胞增殖模型,探讨24-乙酰泽泻醇A对ox-LDL诱导大鼠VSMCs增殖影响的作用机制。不同浓度24-乙酰泽泻醇A（5,10,20 mg·L-1）干预细胞增殖模型12,24,48 h,MTT检测VSMCs增殖情况,Western blot法检测VSMCs PCNA,cyclin D1,cyclin E,p21,p27蛋白表达的变化,RT-PCR检测VSMCs PCNA,p21和p27 mRNA表达情况。MTT结果显示ox-LDL诱导VSMCs的增殖（P〈0.05）;ox-LDL促进VSMCs PCNA,cyclin D1,cyclin E蛋白表达的增加（P〈0.05）,抑制p21,p27蛋白及mRNA的表达（P〈0.05）;24-乙酰泽泻醇A可显著抑制ox-LDL诱导VSMCs的增殖（P〈0.05）及PCNA,cyclin D1,cyclin E蛋白表达,同时提高p21,p27蛋白及mRNA的表达量（P〈0.05）,该现象随着24-乙酰泽泻醇A浓度的增加作用越明显。24-乙酰泽泻醇A可抑制ox-LDL诱导大鼠VSMCs的增殖,其机制可能通过抑制VSMCs细胞周期蛋白（cyclin D1,cyclin E,p21,p27蛋白等）表达有关。
Alisol A 24-acetate,a triterpenoid extracted from Alisma orientale,has shown anti-atherosclerotic actions and many studies have proved that oxidized low density lipoprotein（ Ox-LDL） could promote proliferation of aorta smooth muscle cells（ VSMCs）which are closely related to atherosclerosis（ AS）. The purpose of this study was to evaluate the effect of alisol A 24-acetate on the proliferation of VSMCs isolated from the thoracic aorta of rats induced by ox-LDL. VSMCs were induced by ox-LDL（ 50 mg·L-1） to establish the proliferation model and intervened by alisol A 24-acetate（ 5,10,20 mg·L-1） for 12,24 and 48 h. Then the proliferation of VSMCs was detected by MTT assay; protein expression levels of VSMCs PCNA,cyclin D1,cyclin E,p21,p27 and VSMCs PCNA,p21 and p27 mRNA expression levels were detected by Western blot and Real-time polymerase chain reaction（ RT-PCR） respectively.The results showed that ox-LDL could induce the proliferation of VSMCs（ P 〈 0. 05）,increase the protein expression levels of PCNA,cyclin D1 and cyclin E in the VSMCs（ P 〈 0. 05） and inhibit the protein and mRNA expression levels of p21 and p27（ P 〈 0. 05）. As compared with the model group,alisol A 24-acetate inhibited the proliferation of VSMCs in rats induced by ox-LDL and inhibited the protein expression of VSMCs PCNA,cyclin D1,cyclin E and enhanced the protein and mRNA p21 and p27 expression levels（ P 〈0. 05）. The effect was more obvious with the increase of concentration of alisol A 24-acetate. These data indicate that alisol A 24-acetate can inhibit the proliferation of VSMCs induced by ox-LDL and the mechanism may be associated with inhibiting expression of cyclin protein,including cyclin D1,cyclin E,p21,p27 and so on.
作者
魏伟
周晓茂
汪玉成
林志诚
薛偕华
WEI Wei;ZHOU Xiao-mao;WANG Yu-cheng;LIN Zhi-cheng;XUE Ji-hua(Affiliated Rehabilitation Hospital of Fujian University of Traditional Chinese Medicine, Fuzhou 350003, China;Institute of Fujian Rehabilitation Industry, Fuzhou 350003, China;Fujian University of Traditional Chinese Medicine, Fuzhou 350003, China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2018年第10期2147-2152,共6页
China Journal of Chinese Materia Medica
基金
国家自然科学基金面上项目(81473744,81774380)
福建省科技厅科技平台建设项目(2015Y2001)
