摘要
目的 通过对水溶性3-巯基丙酸（3-MPA）修饰的CdSe-CdS/ZnS核壳结构量子点（3-MPA-QDs）在人脐静脉内皮细胞（HUVEC）中的吸收以及其对细胞增殖和迁移的影响的研究,为量子点光敏剂的应用发展提供理论基础.方法 激光共聚焦扫描显微镜实时对比观察3-MPA-QDs和小分子光敏剂原卟啉IX（PpIX）在HUVEC细胞内的短时（6 h）吸收;观察在48 h内的吸收和对细胞增殖及形态的影响;采用网格培养皿追踪观察24 h内3-MPA-QDs对HUVEC细胞迁移的影响;LysoTrackerTM Deep Red标记溶酶体,采用荧光共定位方法观察3-MPA-QDs的内吞机制.结果 与PpIX的＂上升-饱和＂的吸收方式不同,3-MPA-QDs在6 h内呈持续上升趋势;24 h后细胞内3-MPA-QDs荧光减弱,48 h时荧光更弱,但在新生细胞中可见清晰的3-MPA-QDs荧光信号;迁移实验结果发现,携带3-MPA-QDs的靶细胞在24 h内迁移50μm方格,细胞迁移能力未受到明显影响;荧光共定位结果显示QDs定位于溶酶体.结论 3-MPA-QDs在HUVEC细胞中易进不易出,在胞内定位于溶酶体,但对细胞的增殖和迁移没有明显的影响.
Objective To explore the effects of 3-mercaptopropionic acid （3-MPA） coated CdSe-CdS/ZnS quantum dots （3-MPA-QDs） on the uptake , proliferation and migration of human umbilical vein endothelial cells （HUVEC）, and to study the biotoxicity of 3-MPA-QDs, so as to provide the theoretical basis for the development of quantum dot photosensitizers . Methods The short-term （ 6 h ） uptakes of 3-MPA-QDs and the small molecule photosensitizer protoporphyrin IX （PpIX） in HUVEC cells were real-time observed and compared by a laser confocal scanning microscope. Besides, the uptakes within 48 h as well as the effects of the uptake on morphology and proliferation of HUVEC were also observed. The effect of 3-MPA-QDs on the migration of HUVEC cells within 24 h was observed using a grid dish. LysoTrackerTM Deep Red was used to label lysosomes, and the endocytosis mechanism of 3-MPA-QDs was observed by fluorescence co-localization. Results The fluorescence of 3-MPA-QDs in the HUVEC showed a continuous rising trend within 6 h, weakened after 24 h, and then turned weaker after 48h of the uptake, which is different from the＂up-saturation＂absorption pattern of PpIX. However, the fluorescence signal was still clear and bright which indicate 3-MPA-QDs were passaged into newborn cells. Migration experiments showed that the target cells carrying 3-MPA-QDs migrated 50 μm grids within 24 h, indicating the cell migration ability was not significantly affected. Co-localization results showed that 3-MPA-QDs localized in lysosomes. Conclusions The 3-MPA-QDs localized in lysosome, and they were easy to be absorbed and hard to be excreted by HUVEC. However, no obvious effects of 3-MPA-QDs were observed on cell proliferation and migration.
作者
叶绪英
金文东
石霞飞
阴慧娟
Ye Xuying;Jin Wendong;Shi Xiafei;Yin Huijuan(Department of Cardiology, Tianjin First Central Hospital, Tianjin 300192, China;Institute of Biomedical Engineering, Chinese Academy of Medical Sciences ＆ Peking Union Medical College, Tianjin 300192, China)
出处
《国际生物医学工程杂志》
CAS
2018年第2期160-166,181,共8页
International Journal of Biomedical Engineering
关键词
量子点
人脐静脉内皮细胞
细胞增殖
细胞迁移
溶酶体
Quantum dot
Human umbilical vein endothelial cells
Cell proliferation
Cell migration
Lysosome
