摘要
目的 miR-155在多种肿瘤组织或细胞中过表达,促进淋巴结转移和血管侵犯,抑制细胞凋亡,FoxO1是一种抑癌基因,在多种不同类型的肿瘤中表达下调。本研究旨在探讨miR-155和FoxO1在非小细胞肺癌(non-small cell lung cancer,NSCLC)组织表达水平及其临床意义,以及miR-155和FoxO1的关系。方法收集2014-01-01-2014-11-30台州市中心医院手术切除的NSCLC组织30例作为实验组,距癌组织≥3cm的癌旁组织作为对照组,同时收集临床资料。qRT-PCR检测癌组织和癌旁组织中miR-155和FoxO1表达。建立稳定过表达miR-155的A549和H1975细胞株并用qRT-PCR进行鉴定。使用蛋白质印迹法检测细胞中FoxO1蛋白表达水平。结果 miR-155在NSCLC组织中相对表达量为1.93±0.57,明显高于癌旁组织的1±0.34,差异有统计学意义,t=6.848,P=0.006 2。FoxO1在小细胞肺癌肿瘤组织中相对表达量为1±0.098,癌旁组织中为2.729±0.182,差异有统计学意义,t=8.358,P=0.001 4。miR-155表达水平与TNM分期(P<0.000 1)、淋巴结转移(P<0.000 1)呈正相关。FoxO1表达水平与TNM分期(P=0.000 2)、淋巴结转移(P<0.000 1)呈负相关。癌组织中FoxO1相对表达量与miR-155的表达呈负相关,r=-0.525,P<0.000 1。在miR-155过表达的A549和H1975细胞株中,FoxO1蛋白表达水平与对照组相比明显降低。结论miR-155在NSCLC组织表达水平明显升高,与FoxO1表达水平呈负相关,miR-155可能是通过下调FoxO1基因来促进NSCLC的发展。
OBJECTIVE miR-155 is overexpressed in many kinds of tumors.It has important roles in invasion,metastasis and apoptosis.FoxO1 is a tumor-suppressor gene.FoxO1 is downregulated in many kinds of tumors.This study aimed we investigate miR-155 and FoxO1 expression levels in non-small cell lung cancer（NSCLC）and its clinical significance and the relationship between miR-155 and FoxO1.METHODS Biopsies were collected from 30 NSCLC patients（Jan.1 2014-Nov.30 2014）.Adjacent normal tissues were using as negative control.The expression levels of miR-155 and FoxO1 in patient samples were detected by qRT-PCR.The miR-155 overexpression stable cell lines of A549 and H1975 were made and subsequently tested by qRT-PCR,and the protein levels of FoxO1 in these cells were detected by western blot.RESULTS miR-155 expression level was higher in NSCLC tissues（1.93±0.57）than that in the adjacent normal tissues（1±0.34）,t=6.848,P=0.006 2.FoxO1 expression level was lower in NSCLC tissues（1±0.098）than that in the adjacent normal tissues（2.729±0.182）,t=8.358,P=0.001 4.In NSCLC tumor tissues,miR-155 expression had positive correlation with TNM stage（P〈0.000 1）and lymph node metastasis（P〈0.000 1）,and FoxO1 expression had negative correlation with TNM stage（P=0.000 2）and lymph node metastasis（P〈0.000 1）.It was found that the FoxO1 expression level had midrange negative correlation with the miR-155 expression level in NSCLC tumor tissues,r=-0.525,P〈0.000 1.In the miR-155 overexpression stable cell lines of A549 and H1975,the protein level of FoxO1 was lower than that of the control group.CONCLUSIONS In NSCLC tumor samples,miR-155 is upregulated,and the expression level of FoxO1 have negative correlation with miR-155.The upregulated miR-155 may cause the non-small cell lung cancer progression through FoxO1 downregulation.
作者
杨希
朱君飞
吴旭佳
徐亚妮
YANG Xi;ZHUJun-fei;WU Xu-jia;XUYa-ni(Department of Respiration, Taizhou Center Hospital, Taizhou 318000 ,P. R. China)
出处
《中华肿瘤防治杂志》
CAS
北大核心
2018年第10期713-716,共4页
Chinese Journal of Cancer Prevention and Treatment
