摘要
目的探讨人血管生成素样蛋白4(ANGPTL4)在肝癌中的表达与微血管生成的关系。方法采用免疫组化法检测69例肝癌组织、30例癌旁正常肝组织中ANGPTL4及CD105的表达情况,分析ANGPTL4和CD105标记的微血管密度(MVD)与肝癌临床病理特征的关系。结果 ANGPTL4在肝癌组织中表达明显高于正常肝组织(56.52%vs 20%,P<0.05),在不同年龄、性别、肿瘤直径和包膜浸润的患者中ANGPTL4表达差异无统计学意义(P>0.05);而在是否有门静脉侵袭、淋巴结转移和不同TNM分期的表达差异有统计学意义(P<0.05)。CD105标记的MVD在肝癌组织中表达明显高于正常肝组织(34.82±11.51 vs 15.87±7.52,P<0.05),在不同年龄、性别的患者中CD105的表达差异无统计学意义(P>0.05);在不同肿瘤直径,是否有包膜浸润、门静脉侵袭、淋巴结转移和不同TNM分期中的表达差异有统计学意义(P<0.05)。ANGPTL4阳性组MVD值与阴性组间差异具有统计学意义(t=3.548,P=0.002),ANGPTL4阳性组MVD值较高。结论肝癌中ANGPTL4的表达上调可能参与肿瘤微血管生成、浸润及转移。
Objective To investigate the relationship between angiopoietin like 4（ ANGPTL4） expression and micro-angiogenesis in hepatocellular carcinoma. Methods By immunohistochemistry,the expression of ANGPTL4 and CD105 was detected in 69 hepatocellular carcinoma tissues and 30 normal para-carcinoma hepatocellular tissues. The relationship between clinical pathological characteristics and microvascular density（ MVD） marked with CD105 was analyzed in hepatocellular carcinoma tissues. Results The expression of ANGPTL4 in hepatocellular carcinoma tissues was significantly higher than that in normal hepatocellular tissues（ 56. 52% vs20%,P〈0. 05）,There was no significant difference in ANGPTL4 expression in patients with different age,gender,tumor diameter and cell membrane infiltrates（ P〉0. 05）,but significant difference in different portal vein invasion,lymphatic metastasis and TNM stages（ P〈0. 05）. The expression of CD105 in hepatocellular carcinoma tissues was significantly higher than that of normal hepatocellular tissues（ 34. 82 ± 11. 51 vs 15. 87 ± 7. 52,P〈0. 05）. In hepatocellular carcinoma tissue,the expression of CD105 was not related with age,gender（ P〉0. 05）,but closely related with tumor diameter and cell membrane infiltrates,portal vein invasion,lymphatic metastasis and TNM stages（ P〈0. 05）. The MVD in ANGPTL4 positive expression group was significantly higher than that in ANGPTL4 negative group（ t = 3. 548,P = 0. 002）. Conclusion ANGPTL4 may participate in tumor angiogenesis,invasion and metastasis in HCC.
作者
卫银银
吴方雄
闵亚莉
苗向霞
罗正奇
刘凯歌
WEI Yinyin;WU Fangxiong;MIN Yali;MIAO Xiangxia;LUO Zhengqi;LIU Kaige(Department of Gastroenterology, First Affiliated Hospital of Xi＇ an Medical University, Xi＇ an 710077, China)
出处
《山西医科大学学报》
CAS
2018年第5期457-460,共4页
Journal of Shanxi Medical University
基金
陕西省教育厅科研资助项目(11JK0708)
陕西省教育厅科研资助项目(12JK0704)
