摘要
目的:探讨泛耐药铜绿假单胞菌(PA)群体行为与群体感应系统(QS)基因的相关性。方法:检测PA群体行为包括弹性蛋白酶、绿脓菌素和生物被膜(BF)等和QS基因表达量,分别在泛耐药PA组和全敏感PA组观察PA群体行为与QS基因表达量的相关性。结果:收集泛耐药PA和全敏感PA各5例病例,各10株PA。泛耐药PA组弹性蛋白酶和绿脓菌素与Las I呈正相关关系(P<0.05),BF与RhlR呈正相关关系(P<0.05);全敏感PA组群体行为与QS基因无相关关系(P>0.05)。泛耐药PA组与全敏感PA组第1天收集Rhl I和第14天收集Las I、LasR表达量差异有统计学意义(P<0.05),弹性蛋白酶、BF和Las I、LasR治疗前后表达量变化差异有统计学意义(P<0.05)。1株泛耐药PA发生氨基酸突变。结论:泛耐药PA群体行为与QS基因相关,且弹性蛋白酶、BF和Las I,LasR表达过高,提示QS基因在PA耐药机制中起重要作用。
Objective： To investigate the correlation between bacterial group behavior and expressions of quorum-sensing system（ QS）genes in pan drug resistance Pseudomonas aeruginosa（ PA）. Methods： The bacterial group behaviors（ including elastase,pyocyanin and biofilm） and expression of QS genes were measured. The correlation between PA group behavior and QS gene expression in pan drug resistant PA group and fully sensitive PA group was analyzed. Results： Five cases of pan drug resistance PA and 5 cases of all sensitive PA were collected. In pan drug resistance PA group,the elastase and pyocyanin were positive correlation with expression of Las I（ P〈0. 05）,and the biofilm was positively associated with RhlR（ P〈0. 05）. There was not correlation between bacterial group behavior and QS gene in all sensitive PA group（ P〉0. 05）. The differences of the Rhll level on the first day and Las I and LasR levels on the fourteenth day between two groups were statistically significant（ P〈0. 05）. The differences of the elastase,biofilm,and Las I and LasR levels between before and after treatment were statistically significant（ P〈0. 05）. The amino acid mutation in 1 strain PA was found.Conclusions： The pan drug resistance PA group behavior is correlation with QS gene. The elastase and biofilm,and more expression levels of Las I and LasR hint that the QS gene plays a key role in the drug resistance mechanism of PA.
作者
李镛
刘嘉琳
周莲
LI Yong1,LIU Jia-lin2,ZHOU Lian3(1. Department of Respiratory Medicine,Ruijin Hospital Luwan Branch,Shanghai Jiao Tong University School of Medicine,Shanghai 200020;2. Department of Respiratory Medicine,Ruijin Hospital,Shanghai Jiao Tong University School of Medicine,Shanghai 200025;3. School of Life Sciences and Biotechnology,Shanghai Jiao Tong University,Shanghai 200240,Chin)
出处
《蚌埠医学院学报》
CAS
2018年第3期288-291,295,共5页
Journal of Bengbu Medical College
基金
上海市黄浦区科委课题(HKW201301)
上海市黄浦区卫生系统优秀青年人才培养计划配套课题
关键词
铜绿假单胞菌
耐药
细菌群体行为
群体密度感应系统
Pseudomonas aeruginosa
drug resistance
bacterial group behavior
quorum-sensing system
