摘要
研究香樟种子千粒重的变异水平,不仅有助于香樟的遗传改良,而且有助于香樟产业的发展。现有基于人工计数的香樟种子千粒重测量方法费时费力,而且容易出现误差,尤其不适合大样本的处理。该研究利用扫描仪获取标准图形,通过ImageJ软件自动计数,称重后得到千粒重,并与人工计数法进行了比较;利用该方法对江西境内具有地理代表性和古樟分布广泛的10个种源种子的千粒重进行了测定,分析了其种源及家系间的变异水平。结果表明:图像测定法能快速、准确对香樟种子进行计数,大大提高了千粒重测定的速度;江西局部种源香樟种子千粒重之间的变异主要来自于种源间,群体平均多样性指数为1.10,遗传多样性水平较低,且千粒重性状受地理气候因子影响较小。
The variation research on camphor 1000-seed weight will both contribute to the improvement of geneticbreeding and promote the development of camphor industry.However,the measurement of camphor 1000- seedweight based on artificial counting are time-consuming,laborious and prone to error,especially not suitable for largesample processing.In our research,we use the scanner to obtain the standard graphics,counting automatically by Im.ageJ software,calculating 1000-seed wright after weighing,and compared it with the artificial counting method.Thenthe camphor 1000-seed weight from Jiangxi provenance and its variation were analyzed.The results showed that scan.ner with ImageJ software provides a rapid and accurate way to the camphor seeds counting.1000-seed weight varia.tions among camphor trees from Jiangxi mainly come from the provenance,the average diversity index is 1.10 whichsuggested small genetic variation level among populations,and less affected by climatic factors.
作者
钟永达
周燕玲
李彦强
刘立盘
刘淑娟
杨爱红
刘腾云
余发新
Zhong Yongda et al.(The Key Laboratory of Horticultural Plant Genetic and Improvement of Jiangxi,Institute of Biological Resources,Ji？angxi Academy of Sciences,Nanchang 330096,Chin)
出处
《安徽农学通报》
2018年第10期82-85,共4页
Anhui Agricultural Science Bulletin
基金
江西省科技支撑计划项目(20151BBF60018)
江西省林业厅林业科技创新专项(201701)
江西省科学院引进博士项目(2014-YYB-02)
江西省科学院普惠项目(2014-XTPH1-02)
南昌市优势科技创新团队建设计划项目(2015CXTD005)
关键词
香樟
千粒重
ImageJ软件
种源
变异
Cinnamomum camphora
1000-seed weight
ImageJ analysis software
Provenance
Variation
