摘要
目的:观察脑微透析探针置入大鼠海马后组织间液葡萄糖、乳酸水平的动态变化,以探讨合适的探针置入平衡期。方法:使用微透析系统在探针置入大鼠海马后,于自由活动状态下连续取样,每20 min取样一次,连续进行5 h,并检测葡萄糖、乳酸水平变化。结果:海马葡萄糖和乳酸水平在探针置入后都出现升高,探针置入20 min后,细胞间液葡萄糖、乳酸水平分别为462.92±96.45μmol/L、3.28±0.77 mmol/L,置入5 h后葡萄糖、乳酸水平分别为257.58±44.54μmol/L、1.36±0.28 mmol/L。结论:脑微透析探针的置入可引起大鼠海马葡萄糖、乳酸水平升高,在进行实验取样时探针置入后应至少应选择60 min作为平衡期。
Objective： To observe the effect of cerebral microdialysis probe implantation on the levels of glucose and lactate in rat hippocampal interstitial fluid, and investigate the appropriate equilibrium phase after probe implantation. Methods： Take microdialy- sis sample in the rat hippocampus for 5 hours, 20min per sample, after cerebral microdialysis probe implantation, test and analyse the levels of glucose and lactate. Results： The levels of glucose and lactate are increased after the probe implantation, the levels of glucose and lactate in hippocampal intercellular fluid 20 min after probe implantation are 462. 92 ± 96.45 μmol/L, 3.28± 0. 77 mmol/L, and 257. 58 ±44. 54 μmol/L, 1.36±0. 28 mmol/L after 5 hours. Conclusion： The cerebral microdialysis probe implantation has an increase effect on the levels of glucose and lactate in rat hippoeampus, no less than 60 min should be choosen for equilibrium phase after the cerebral mierodialysis probe implantation.
作者
刘奇
杜萍
施晓兰
邢文文
卢圣锋
张承舜
尹海燕
吴巧凤
余曙光
Liu Qi;Du Ping;Shi Xiaolan;Xing Wenwen;Lu Shengfeng;Zhang Chengshun;Yin Haiyan;Wu Qiaofeng;Yu Shuguang(Acupuncture and Tuina School, Shaanxi University of traditional Chinese Medicine, Xi＇an, 712046, China;Acupuncture and Tuina School/The 3~ Teaching Hospital, Chengdu University of Traditional Chinese Medicine, Chengdu, 610075, China;School of Basic Medical Sciences, Shaanxi University of Traditional Chinese Medicine, Xi an, 712046, China;Key Laboratory of Acupuncture and Medicine Research of Ministry of Education, Nanjing University of Traditional Chinese Medicine, Nanjing, 210023, China.)
出处
《成都中医药大学学报》
2018年第2期8-10,37,共4页
Journal of Chengdu University of Traditional Chinese Medicine
基金
国家自然科学基金(81202743)
陕西中医药大学科研基金(2016PY01)
陕西省自然科学基础研究计划(2017JQ8020)
关键词
脑微透析
平衡期
葡萄糖
乳酸
Cerebral microdialysis
Equilibrium phase
Glucose
Lactate
