摘要
目的 BMI1基因在胃肠道间质瘤(GIST)发生发展中的作用尚未明确。文中探讨BMI1基因在GIST组织中的表达,并分析其与临床病理学特征的关系。方法回顾性分析2012年8月至2015年10月就诊于南宁市第一人民医院68例GIST患者资料。采用免疫组化法检测BMI1在正常胃肠道组织和GIST组织中的表达,分析BMI1基因与GIST的各项临床病理参数之间的关系。采用Western印迹法检测BMI1蛋白的表达。结果 GIST组织中BMI1阳性表达率显著高于非GIST组织(76.47%vs 36.84%,P<0.05)。BMI1基因的蛋白表达情况在不同危险度分组之间比较的差异有统计学意义(P<0.05),且在高危险度组中阳性表达率最高(93.75%);而在不同性别、年龄、发生部位、组织学类型及是否转移组间比较差异均无统计学意义(P>0.05)。BMI1阳性组肿瘤组织中增殖基因PCNA、Cyclin D1 mRNA的表达量高于BMI1阴性组,促凋亡基因Caspase-7、Smac mRNA的表达量低于BMI1阴性组,抗凋亡基因Livin、p53、Bcl-2 mRNA的表达量高于BMI1阴性组(P<0.05)。结论BMI1蛋白在GIST组织中的表达增加,其表达量与GIST的危险度分级相关。
Objective The role of BMI1 gene in the development of gastrointestinal stromal tumor（ GIST） has not yet been clarified. This study aimed to explore the expression of BMI1 gene in gastrointestinal stromal tumor,and analyze its relationship with clinical pathological features of GIST. Methods The clinical data of 68 GIST patients treated in The First People＇s Hospital of Nanning from August 2012 to October 2015 were analyzed retrospectively. The expression of BMI1 in normal gastrointestinal tissues and GIST tissues were detected with immunohistochemistry method,and analyzed the relationship between various clinicopathological parameters of GIST and BMI1. The expression of BMI1 protein was detected by Western blot. Results The positive rate of BMI1 was much higher in GIST group than in non-GIST（ 76.47% vs 36.84%,P〈0.05）. The difference in the expression of BMI1 protein between the different risk groups was statistically significant（ P〈0. 05）. The positive expression rate was the highest in the high-risk group（ 93.75%）,but had no statistically significant difference among different genders,age,locations,histological types and whether metastasis（ P〈0.05）. Expression of proliferation genes such as PCNA,Cyclin D1 mRNA in BMI1 positive group were higher than those in BMI1 negative group,the expression of Pro-apoptotic genes such as Caspase-7,Smac mRNA were lower than those in BMI1 negative group,the expression of anti-apoptosis genes such as Livin,p53,Bcl-2 mRNA were higher than those in BMI1 negative group（ P〈0.05）.Conclusion The expression of BMI1 protein was increased in GIST tissue. It is correlated with the risk classification,and is an important factor affecting the prognosis of patients.
作者
许国定
蒋洪棉
朱茂光
朱世龙
苏泽
李春玲
黄海
XU Guo-ding;JIANG Hong-mian;ZHU Mao-guang;ZHU Shi-long;SU Ze;LI Chun-ling;HUANG Hai(Department of HGS;Department of Pathology, First People＇s Hospital of Nanning , Nanning 530022, Guangxi , China)
出处
《医学研究生学报》
CAS
北大核心
2018年第6期631-635,共5页
Journal of Medical Postgraduates
基金
广西壮族自治区卫生和计划生育委员会自筹经费科研课题项目(Z2015268)
