摘要
目的建立不同成熟度南五味子中6种主要木脂素类成分的同时定量方法,并基于化学模式识别技术,对不同成熟度南五味子进行分类,为南五味子合理采收加工提供科学依据。方法采用超高效液相色谱(UPLC)法建立不同成熟度五味子中6种木脂素类化合物的同时定量方法,色谱条件为ACQUITY UPLC BEH C18(50 mm×2.1 mm,1.7μm)色谱柱,以乙腈-0.1%甲酸水为流动相梯度洗脱,体积流量0.4 m L/min,检测波长254 nm,柱温35℃。同时以南五味子的指标成分五味子酯甲为监测因子,采用主成分分析(PCA)和分层聚类法(HCA)对不同成熟度的南五味子样品进行聚类分析。结果 6种木脂素类化合物在1.257~156.250μg/m L线性关系良好(r<0.999),重复性、稳定性、精密度试验RSD均<3%。根据不同成熟度主要成分的变化,PCA和HCA分析将6个不同成熟度的五味子及混合样品主要分为3类:60%以下红,70%红以及全红的样品。结论 60%红样品的木脂素类化合物具有较高含量,说明基于UPLC技术的聚类分析及PCA联合分析方法能够较好地区分不同成熟度的南五味子药材质量,为其质量控制和采收期选择提供了技术支持和科学依据。
Objective To establish the simultaneous determination method of six lignans from Schisandra sphenanthera and classify the different maturity of Schisandra sphenanthera via chemical pattern recognition approach. Methods The simultaneous determination method was established by UPLC technology, the samples were performed by Acquity UPLC BEH C18（50 mm × 2.1 mm, 1.7 μm） and eluted with acetonitrile and 0.1% formic acid, the flow rate was 0.4 m L/min, column temperature was 35 ℃, and the detection wavelength was 254 nm. The collected data were analyzed and classified by principal component analysis（PCA） and hierarchical cluster analysis（HCA） that the Schisantherin was selected as the monitoring component. Results Six lignans were in good linear relationship with the linear range 1.257—156.250 μg/m L（r 〈0.999）; Moreover, the RSD values of the repeatability, stability, and precision test were all less than 3%. According to the variation of main components in different grades of maturity, six different grades of maturity of S. sphenanthera were divided into three groups： 60%-mature sample, 70%-mature sample, and mature sample. Conclusion Cluster analysis based on UPLC technology and PCA joint analysis method are simple, rapid, sensitive, and accurate, which provides technical support and scientific basis for the selection of quality control and harvest time.
作者
吕杨
刘妍如
唐志书
王梅
宋忠兴
孔馨逸
江大海
钱大玮
LV Yang;LIU Yan-ru;TANG Zhi-shu;WANG Mei;SONG Zhong-xing;KONG Xin-yi;JIANG Da-hai;QIAN Da-wei(Shaanxi Province Key Laboratory of New Drugs and Chinese Medicine Foundation Research, Shaanxi Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Shaanxi University of Chinese Medicine, Xianyang 712083, China;Collaborative Innovation Center of Chinese Medicinal Resources Industrialization of Jiangsu Province, Jiangsu Key Laboratory for Traditional Chinese Medical Formular Research, Nanjing University of Chinese Medicine, Nanjing 210023, China)
出处
《中草药》
CAS
CSCD
北大核心
2018年第9期2147-2152,共6页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(81501229)
陕西省教育厅自然科学专项项目(15JK1200)
关键词
南五味子
超高液相色谱法
模式识别
主成分分析
成熟度
Schisandra sphenanthera Rehd. et Wils.
UPLC
pattern recognition
PCA
grade of maturity
