摘要
目的探讨髓系细胞触发受体－1（TREM－1）rs2234237 A/T、rs9471535 A/G基因多态性与冠状动脉粥样硬化性心脏病（简称冠心病）易感性的关联。方法病例对照研究。于2016年11月至2017年4月在荆州市中心医院选取120例冠心病患者（冠心病组）、90名健康体检者（正常对照组）。采用Sanger法对所有受试者TREM－1基因（rs2234237和rs9471535）单核苷酸多态性（SNPs）分析。比较两组基线临床资料和基因型分布频率。非条件Logistic回归分析TREM－1基因（rs2234237和rs9471535 ）多态性与冠心病易感性的关系。结果两组性别比例、年龄、体重指数、总胆固醇、甘油三酯和低密度脂蛋白胆固醇水平差异均无统计学意义（分别为χ^2 ＝0.575，P〉0.05；t＝－1.670，P〉0.05；t＝－1.719，P〉0.05；t＝1.011，P〉0.05；t＝－1.834，P〉0.05；t＝0.474，P〉0.05），而吸烟、高血压和糖尿病比例与高密度脂蛋白胆固醇及空腹血糖水平差异有统计学意义（分别为χ^2 ＝4.321，P〈0.05；χ^2 ＝39.213，P〈0.01；χ^2 ＝24.184，P〈0.01；t＝5.476，P〈0.01；t＝－5.106，P〈0.01） 。TREM－1 rs2234237、rs9471535基因型及等位基因分布在两组间的差异有统计学意义（rs2234237：分别为χ^2 ＝6.893，P〈0.05；χ^2 ＝7.159，P〈0.05。rs9471535：分别为χ^2 ＝8.284，P〈0.05；χ^2 ＝8.314，P〈0.05）。在冠心病组中，rs2234237多态性位点的（AT＋TT）基因型频率显著低于对照组（38.3%与53.3%，χ^2 ＝4.680，P＝0.031），rs9471535多态性位点的（AG＋GG）基因型频率显著低于对照组（37.5%与53.3%，χ^2 ＝5.225，P＝0.022），此外，冠心病组rs2234237多态性位点的T等位基因频率显著低于对照组（21.7%与33.3%，χ^2 ＝7.159，P＝0.007），rs9471535多态性位点的G等位基因频率显著低于对照组（20.8%与33.3%，χ^2 ＝8.314，P＝0.004）。rs2234237 TT携带者患冠心病的风险是AA的0.173倍（
Objective To explore the relationship between gene polymorphisms of triggering receptor expressed on myeloid cells-1 （TREM-1） rs2234237A/T, rs9471535A/G and susceptibility to coronary atherosclerotic heart disease（coronary heart disease for short, CHD）.Methods A case-control study. 120 patients with CHD （CHD group） and 90 healthy people （Normal control group） were selected from November 2016 to April 2017 in Jingzhou Central Hospital. The single nucleotide polymorphisms（SNPs） of TREM-1gene （rs2234237 and rs9471535）were analyzed using Sanger method in all subjects. Comparing baseline clinical data and the distribution of genotype frequencies in the two groups. Non conditional logistic regression was used to analyze the relationship between TREM-1 gene （ rs2234237 and rs9471535） polymorphisms and susceptibility to CHD.Results The proportion of gender as well as level of age, body mass index, total cholesterol, triglyceride and low density lipoprotein cholesterol were not statistically significant between the two groups（χ^2 =0.575, P〉0.05; t=-1.670, P〉0.05; t=-1.719, P〉0.05; t=1.011, P〉0.05; t=-1.834, P〉0.05; t=0.474, P〉0.05, respectively）, while the proportion of smoking, hypertension and diabetes as well as level of high density lipoprotein cholesterol and fasting plasma glucose were statistically significant between the two groups （χ^2 =4.321, P〈0.05; χ^2 =39.213, P〈0.01; χ^2 =24.184, P〈0.01; t=5.476, P〈0.01; t=-5.106, P〈0.01, respectively）. The distribution of rs2234237, rs9471535 genotypes and alleles was statistically significant in the two groups （rs2234237： χ^2 =6.893, P〈0.05; χ^2 =7.159, P〈0.05, respectively; rs9471535： χ^2 =8.284, P〈0.05; χ^2 =8.314, P〈0.05, respectively）. The genotype frequency of rs2234237（AT＋ TT）in CHD group was significantly lower than in the control group（38.3% vs 53.3%, χ^2 =4.680, P=0.031）, and the genotype frequency of rs9471535（AG＋ GG） in CHD group was significantly lower than in the
作者
陆娇
李承彬
邓明凤
李滔
朱静
Lu Jiao;Li Chengbin;Deng Mingfeng;Li Tao;Zhu Jing(Clinical Laboratory Diagnostics Profession, the Second Clinical Medical College, Yangtze University, Jingzhou 434020, Chin)
出处
《中华检验医学杂志》
CAS
CSCD
北大核心
2018年第6期436-441,共6页
Chinese Journal of Laboratory Medicine
基金
荆州市医疗卫生科技计划项目(2016-83)
关键词
冠心病
髓系细胞触发受体-1
多态性
单核苷酸
疾病易感性
Coronary disease
Triggering receptor expressed on myeloid cells-1
Polymorphism
single nucleotide
Disease susceptibility
