摘要
目的:对艾塞那肽复乳在动物体内降血糖作用进行研究,并考察其皮下注射刺激性。方法:以生理盐水、空白复乳为空白对照,市售艾塞那肽注射剂为阳性对照,考察艾塞那肽复乳对正常小鼠的降血糖作用;制备大鼠2型糖尿病模型,考察艾塞那肽复乳的降血糖效果;注射局部观察及HE染色切片观察皮下注射的刺激性。结果:对正常小鼠,艾塞那肽注射剂给药后90 min血糖降至最低,120 min时血糖开始逐渐上升,艾塞那肽复乳在150 min内可持续降低血糖;给药150 min取血后灌胃给予葡萄糖溶液,注射剂组60 min后血糖开始升高,而艾塞那肽复乳组血糖仍保持在较低水平。对2型糖尿病大鼠,注射剂组停药7 d后,血糖水平显著回升(P<0.05),艾塞那肽复乳组血糖仍维持较低水平;艾塞那肽复乳皮下注射对局部皮肤及组织均无明显或不可逆的影响。结论:与艾塞那肽注射剂相比,艾塞那肽复乳在动物体内具有稳定而长效的降血糖作用,局部应用安全性较高。
Objective： To study the hypoglycemic effect and the hypodermic injection irritation of exendin multiple emulsion in testing animals. Methods： Using normal saline and blank multiple emulsion as the blank controls and the marketed exendin injection as the positive control,the hypoglycemic effects of exendin multiple emulsion in healthy mice and mice with gastric administration of 10%glucose solution were studied. Type II diabetes rat model was established,and the hypoglycemic effect of exendin multiple emulsion was investigated. The injection of local skin was observed and the slides of injection tissue were studied after HE staining to evaluate the irritation of exendin multiple emulsion. Results： In the healthy mice,the blood glucose reached the lowest point at 90 min for exendin injection and rose gradually at 120 min,while the blood glucose maintained low levels during the testing period for exendin multiple emulsion. After the gastric administration of glucose solution,the blood glucose increased at 60 min for exendin injection,while that kept low levels for exendin multiple emulsion. In type II diabetes rats,the blood glucose increased gradually after 7-day withdrawal（ P〈0. 05）,while that kept low levels for exendin multiple emulsion. The hypodermic injection of exendin multiple emulsion showed no significant or irreversible irritation to the local skin and tissue. Conclusion： Compared with exendin injection,exendin multiple emulsion exhibits stable and sustained hypodermic effects with high local use safety.
作者
杜修桥
王军
邢俊俏
Du Xiuqiao;Wang Jun;Xing Junqiao(Department of Pharmacy, the First People＇ s Hospital of Zaoyang City, Hubei Zaoyang 441200, China;Renmin Hospital of Wuhan University;Institute of Pathological Diagnosis, Jianghan University)
出处
《中国药师》
CAS
2018年第6期989-991,共3页
China Pharmacist
关键词
艾塞那肽
复乳
2型糖尿病
降血糖
Exendin
Multiple emulsion
Type II diabetes
Hypodermic effect
