摘要
目的:研究甲基苯丙胺依赖不同时间对大鼠脑纹状体内G蛋白信号调节因子4(regulator of G-protein signaling 4,RGS4)和代谢型谷氨酸受体5(metabotropic receptor 5,m GluR5)信号传导的影响。方法:建立大鼠甲基苯丙胺(methamphetamine,METH)依赖一周组(1W)、两周组(2W)模型,Western blot技术检测大鼠纹状体内RGS4、m GluR5、G蛋白α亚单位蛋白Q(Gαq)、磷脂酶C-β1(phospholipase C-β,PLCβ1)表达水平。结果:METH依赖1W、2W组伴药箱停留时间比生理盐水对照组增加,提示METH依赖模型建成。模型组大鼠纹状体内RGS4蛋白表达水平较生理盐水对照组相比明显下调,而模型组m GluR5蛋白和Gαq蛋白、PLCβ1蛋白表达与生理盐水对照组比较均有不同程度上调,且2W组较1W组改变更明显。结论:METH给药可能导致大鼠脑纹状体内RGS4表达下调,m GluR5表达上调,还可使与m GluR5偶联的Gαq、PLCβ1表达均出现不同程度上调。且METH依赖时间越长,RGS4、m GluR5、Gαq、PLCβ1的表达水平变化越显著。
AIM： To investigate whether regulator of G-protein signaling 4（ RGS4） and metabotropic receptor 5（ mGluR5） expression level changed in striatum of the methamphetamine（ METH）-dependent rat and whether the changes were related to the time of METH treatment.METHODS： METH-dependent rat model of one week and two weeks were established. The expression levels of RGS4,mGluR5,Gαq,PLCβ1 were detected by Western blot analysis. RESULTS： Rats treated with METH of one week and two weeks spent much more time in the METH-paired chamber than NS control group,suggested that METH-dependent model has successfully established. Compared with NS control group,the expression level of RGS4 in the striatum of rats in model group was down-regulated remarkably. Furthermore,the expression level of mGluR5 and its coupling protein Gαq, phospholipase C-β1（ PLCβ1） were up-regulated in different degrees when compared with NS control group,and the change is more remarkable in two weeks group than that of one week group. CONCLUSION：METH treatment down regulate the RGS4 and up regulate mGluR5 in rat striatum,and the expression level of mGluR5 coupling protein Gαq,PLCβ. And the longer the time of METH-dependent,the more significant the changes of RGS4,mGluR5,Gαq,and PLCβ1.
作者
赵林波
隋念含
李利华
洪仕君
邢豫明
赵永娜
ZHAO Linbo;SUI Nianhan;LI Lihua;HONG Shijun;XING Yuming;ZHAO Yongna(School of Institute of Information Technology, Yunnan University Of TCM, Kunming 650500, Yunnan, China;School of Pharmaceutical Sciences and Yunnan Key Laboratory of Pharmacology for Natural Product, Kunming Medical University, Kunming 650500, Yunnan , China;School of Forensic Medicine, Kunming Medical U- niversity,Kunming 650500, Yunnan, China;Key Laboratory of Narcotics Technology and Ministry of Police of Yunnan Provincial Police Department, Kunming 650228, Yunnan, China)
出处
《中国临床药理学与治疗学》
CAS
CSCD
2018年第5期536-540,共5页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
云南省应用基础研究计划联合专项基金资助项目(2012FB023)
