摘要
目的研究红芪多糖(HPS)对糖尿病心肌病(DCM)db/db小鼠心肌细胞凋亡相关基因Bcl-2相关X蛋白(Bax)、B细胞淋巴瘤/白血病-2(Bcl-2)和半胱氨酸天冬氨酸蛋白酶3(Caspase-3)蛋白及基因表达的影响。方法用随机数表法将db/db小鼠分为5组:高、中、低3个剂量实验组、对照组和模型组。正常组为12只同周龄同背景db/m小鼠。高、中、低3个剂量实验组分别给予HPS 200,100,50 mg·kg^(-1)·d^(-1)混悬液灌胃;对照组给予670μg·mL^(-1)罗格列酮混悬液4 mg·kg^(-1)灌胃;模型组给予同等剂量0.9%Na Cl 0.2 mL·d^(-1)灌胃;正常组不干预。连续干预8周。于给药前及给药后第2,4,6,8周末,以免疫印迹法检测心肌组织Bax、Bcl-2以及Caspase-3蛋白表达。结果经HPS干预8周后,对照组、模型组和高、中2个剂量实验组的Bax蛋白水平分别为0.61±0.04,1.04±0.02,0.56±0.16,0.75±0.11;这4组的Bcl-2蛋白水平分别为0.87±0.03,0.40±0.07,1.04±0.06,0.72±0.05;这4组的Caspase-3蛋白水平分别为0.44±0.05,0.78±0.11,0.28±0.05,0.63±0.12,模型组与正常组比较,差异均有统计学意义(均P<0.01);高、中2个剂量实验组与模型组比较,差异均有统计学意义(均P<0.01)。结论 HPS可以增加糖尿病心肌病db/db小鼠心肌组织中Bcl-2蛋白表达、而降低Bax与Caspase-3的蛋白表达。
Objective To explore the effect of Hedysarum polybotrys polysacchcaide（ HPS） on cardiomyocyte apoptosis of db/db mice with diabetic cardiomyopathy（ DCM） by taking B-cell leukemia/lymphoma2（ Bcl-2）,Bcl-2 assaciated X protein（ Bax） and cysteinylaspartate specific proteinase（ Caspase-3） as the indexes. Methods Male db/db mice were randomly divided into five groups： experimental-L,-M,-H groups,control group and model group,additional 12 non transgenic db/m male mice with the same background were assigned into the normal group. The experimental-H,-M,-L groups were given intragastric administration of HPS 200,100,and 50 mg kg^（-1）·d^（-1） respectively; the control group was given intragastrically by 670 μg·m L^（-1） rosiglitazone suspension 4 mg·kg^（-1）; the model group was given intragastric administration of the same dose of 0. 9% Na Cl 0. 2 m L·d^（-1）; the normal group did not intervene. The mice of all groups were successive for 8 weeks. The expressions of Bcl-2,Bax and Caspase-3 protein in cardiac muscle tissue were measured by Western blot. Results Eight weeks after treatment,the protein expressions of Bax of mouse in control group,model group and experimental-H,-M groups were 0. 61 ± 0. 04,1. 04 ± 0. 02,0. 56 ± 0. 16,0. 75 ± 0. 11; the protein expressions of Bcl-2 of mouse in the four groups were 0. 87 ± 0. 03,0. 40 ± 0. 07,1. 04 ± 0. 06,0. 72 ± 0. 05; the protein expressions of Caspase-3 of mouse in the four groups were 0. 44 ± 0. 05,0. 78 ± 0. 11,0. 28 ± 0. 05,0. 63 ± 0. 12. Model group decreased significantly compared with normal group（ all P〈0. 01）. Experimental-H,-M groups decreased significantly compared with model group（ all P〈0. 01）.Conclusion HPS can increase the protein expression of Bcl-2 and reduce the protein expression of Bax and Caspase-3 in myocardial tissue of db/db mice with DCM.
作者
王栋
金智生
何流
张花治
王东旭
和彩玲
楚惠媛
WANG Dong;JIN Zhi - sheng;HE Liu;ZHANG Hua - zhi;WANG Dong - xu;HE Cai - ling;CHU Hui - yuan(Department of Chinese Internal Medicine, College of Clinical Traditiortal Chinese Medicine, Gansu University of Traditional Chinese Medicine, Lanzhou 730000, Chin)
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2018年第11期1330-1333,共4页
The Chinese Journal of Clinical Pharmacology
基金
国家自然科学基金地区科学基金资助项目(81360538)
关键词
糖尿病心肌病
细胞凋亡
红芪多糖
DB/DB小鼠
diabetic cardiomyopathy
cardiomyocyte apoptosis
Hedysarum polybotrys polysacchcaide
db/db mice
