摘要
为建立以色列急性麻痹病毒（Israeli acute paralysis virus，IAPV）特异性核酸检测方法，本研究以IAPV polvmerase polyprotein基因为靶基因设计合成1对特异性引物，建立了IAPV的RTPCR检测方法。结果显示，对蜜蜂的5种不同病毒进行RT-PCR检测，只有IAPV病毒检测结果为阳性，具有良好的特异性；对IAPV的最低检测限为10^3拷贝／μL，具有较高的灵敏度；利用该检测方法对来自福建省不同养蜂场的8份蜜蜂样品进行检测，3份样品为阳性，通过序列比对分析，同源性超过99％，进一步证实为IAPV。结果表明，所建立的IAPV RT-PCR检测方法灵敏度高、特异性强、重复性好，具有良好的实用性。
To establish a rapid assay for Israeli acute paralysis virus（IAPV）, RT-PCR method was developed targeting polymerase polyprotein gene of IAPV. The results showed that the test for 5 different viruses of bees with RT-PCR method, only IAPV test was positive, indicating that the method had high specificity. In addition, the sensitivity of RT-PCR was 103 copies/μL. Furthermore,a total 3 positive samples for IAPV were detected from 8 bees samples of different apiary by the RT-PCR method, at the same time, sequence alignment analysis showed the homology was more than 99%, which showed that the method had good practicability. Therefore, the RT-PCR method provided a novel rapid, sensitive and good repeatability detection method for IAPV infection.
作者
张体银
李丹丹
郑腾
白泉阳
张志灯
王武军
于师宇
ZHANG Ti-yin;LI Dan-dan;ZHENG Teng;BAI Quan-yang;ZHANG Zhi-deng;WANG Wu-jun;YU Shi-yu(Fujian Provincial Key Laboratory of Inspection and Quarantine Technology Research, Fujian Entry-Exit Inspection and Quarantine Bureau, Fuzhou 350001, China;Technical Center of Hainan Entry-Exit Inspection and Quarantine Bureau, Haikou 570311, China)
出处
《中国兽医学报》
CAS
CSCD
北大核心
2018年第6期1105-1108,共4页
Chinese Journal of Veterinary Science
基金
福建省科技计划重点资助项目(2014N0001)
福建检疫局科技计划资助项目(FK2015-JS001)
海南省社会发展科技专项资助项目(2015SF29)
