摘要
目的 模拟临床创伤休克并发脓毒性休克的情况，建立＂二次休克＂打击动物模型，连续观察小鼠血压和生存情况，为筛选抗休克药物提供实验动物模型。方法 采用双后肢股骨骨折加眼球放血45%建立创伤休克（TH）模型；于TH术后8h进行盲肠结扎穿孔术（CLP）构建＂二次打击＂模型。将70只6~8周龄成年雄性C57BL/6小鼠随机分为：创伤休克组（TH＋生理盐水），模型组（TH＋CLP＋生理盐水），假手术组（TH＋开腹腔＋生理盐水），各组持续观察小鼠平均动脉血压（MPa）以及生存情况；于TH后0 h，2 h，32 h，各组分别处死5只小鼠（处死小鼠前持续观察小鼠生命状态），收集血液检测血清肌酐值。结果 各组TH后血压急剧下降，在第二次打击CLP后血压持续降低，模型组血压下降较假手术组与创伤休克组剧烈（P〈0.05）。与假手术组、创伤休克组相比，模型组死亡率明显增加（P〈0.05），血清肌酐值显著增高（P〈0.05），经CLP后小鼠的呼吸频率增加，渐出现体温冰冷，蜷缩拥挤。结论 小鼠TH术后8 h并发脓毒性休克（即＂二次休克＂模型）较假手术组与创伤休克组的死亡率明显增加，血压持续下降，肾功能损害，符合临床创伤性休克并发脓毒性休克的病理生理改变，动物模型成功构建。
Objective To simulate the situation of clinical traumatic shock combined with septic shock. The ＂secondary shock＂ animal model was established to continuously observe the blood pressure and survival of mice, and to provide an experimental animal model for screening anti-shock drugs. Methods A model of traumatic shock （TH） was established using femoral fractures in both hindlimbs plus 45% of ocular exsanguination. Cecal ligation and puncture （CLP） was performed 8 hours after TH to establish a ＂second strike＂ model. 70 male C57BL/6 mice aged 6 to 8 weeks were randomly divided into traumatic shock group （TH ＋ saline）, model group （TH ＋ CLP ＋ saline）, and sham operation group （TH ＋ open celiac ＋ normal saline）. The mean arterial blood pressure （MPa） and survival of the mice were observed continuously in each group; at TH 0h, 2h and 32h, 5 mice were sacrificed respectively in each group （the mice were continuously observed before the mice were sacrificed）, and the blood was collected to detect serum creatinine. Results Each group of blood pressure （BP） dropped sharply after TH and the BP continued to decrease after CLP. The BP of model group decreased more severe than the sham operation group and the traumatic shock group （P〈0.05）. Compared with the sham-operated group and the traumatic shock group, the mortality rate of the model group was significantly increased （P〈0.05）, and the serum creatinine value was significantly higher （P〈0.05）. After the CLP, the respiratory frequency of the mice increased, and body temperature gradually declined. Conclusions Compared with the sham-operated group and the traumatic shock group, the mortality of mice in secondary shock model increased significantly, blood pressure continued to decrease, and renal function was impaired, which conformed to clinical traumatic shock process. The animal model of ＂Secondary Shock＂ was successfully established.
作者
唐婉琦
马晓媛
杨雪
马娓
严军
梁华平
Tang Wanqi;Ma Xiaoyuan;Yang Xue;Ma Wei;Yan Jun;Liang Huaping(State Key Laboratory of Trauma, Burns and Combined Injury, Research Institute of Surgery, Daping Hospital, Army Military Medical University, Chongqing, Chin)
出处
《实用休克杂志（中英文）》
2018年第2期91-93,103,共4页
Journal of Practical Shock
基金
国家自然科学基金(项目号81671906)
