摘要
利用Illumina Hi Seq高通量测序技术,对我国南方湿热地区某O_3/BAC饮用水处理过程中各工艺出水及滤料中微生物多样性及其变化规律进行研究。结果表明,测序共获得385 499条有效数据(Effective Tags),归类为45个门、86个纲、165个目、308个科、567个属。微生物多样性随工艺流程整体呈下降趋势,尤其是消毒工艺能大幅削减水体中的微生物,消毒效果较好,但生物活性炭池出水中微生物多样性较V型滤池出水(FB)不降反增,且滤砂(FS)和活性炭上均有大量微生物附着,其中活性炭上附着的微生物多样性高于原水。此外,所有样品中变形菌门、放线菌门、拟杆菌门、厚壁菌门和浮霉菌门含量较高,分别为68.47%、10.64%、6.15%、4.63%和1.51%;鞘氨醇单胞菌属在FB、FS和BAC样品中大量滋生,且与假单胞菌属在出厂水中均有微量存在,二者可引起管网腐蚀,是饮用水安全的一大隐患,应予以重视。
The Illumina HiSeq high-throughput sequencing technology was used to study the mi- crobial diversity and variation in the effluent from each unit and in the filter media of an O_3/BAC drink- ing water treatment process, located in a southern humid heat area of China. A total of 385 499 effective tags were sequenced, which were classified as 45 phylum, 86 class, 165 order, 308 family, and 567 genera. The microbial diversity decreased with the whole process. Especially, the microbial diversity was reduced greatly in the disinfection process, which indicated high disinfection efficiency. However, the microbial diversity in the effluent of biological activated carbon filter was larger than that of the V-shaped sand filter. In addition, the filtration sand and the activated carbon were attached by a large amount of microorganisms, and the microbial diversity in the activated carbon was larger than that of raw water. Mo- reover, the contents of Proteobaeteria, Actinobacteria, Baeteroidetes, Firmicutes and Planetomycetes were relatively high in all samples, accounting for 68.47% , 10.64% , 6.15% , 4.63% and 1.51% , respec- tively. A large number of Sphingomnas were detected in the sample of FB, FS and BAC, and were found together with Pseudomonas in finished water, both of which could cause corrosion of the pipe network and were great potential risks to the drinking water.
作者
周常
蔡广强
张金松
黄河洵
ZHOU Chang;CAI Guang-qiang;ZHANG Jin-song;HUANG He-xun(School of Civil Engineering, Guangzhou University, Guangzhou 510006, China;Shenzhen Water Affairs 〈 Group 〉 Co. Ltd., Shenzhen 518031, China;School of Civil and Environmental Engineering, Harbin Institute of Technology 〈 Shenzhen 〉 , Shenzhen 518055, China;Key Laboratory of Water Resource Utilization and Environmental Pollution Control in Shenzhen, Shenzhen 518055, China)
出处
《中国给水排水》
CAS
CSCD
北大核心
2018年第11期45-50,共6页
China Water & Wastewater
基金
国家水体污染控制与治理科技重大专项(2015ZX07406-004)
深圳市水务(集团)有限公司内部课题(2016YT12)