摘要
目的探讨蒙脱石对肝脏创面止血的有效性与安全性。方法 36只SD大鼠建立肝脏出血模型后随机分为三组:A组采用蒙脱石0.05g处理出血创面,C组采用壳聚糖止血粉0.05g处理,B组为空白对照,每组12只;记录创面出血完全停止时间。体外预实验:取大鼠眼眶处血1ml,移入枸橼酸钠抗凝管中,亦随机分为蒙脱石(A组)、空白对照(B组,不加任何药物)和壳聚糖止血粉处理(C组)三组,每组12管;记录血液凝固时间。1周后检测肝功能和炎症因子水平,并采用HE染色观察肝脏愈合组织的病理变化。结果与B组相比,A组和C组体外和体内凝血时间均缩短(P<0.05),ALT、AST、IL-1、IL-6、IL-8水平均降低(P<0.05)。HE染色结果显示,各组肝脏愈合良好,蒙脱石未对肝脏造成明显的不良反应。结论蒙脱石有显著的促进创面止血功能,且组织安全性良好。
Objective To investigate the hemostatic effect and safety of montmorillonite powder in liver hemorrhagic wound.Methods The liver hemorrhage model was established in 36 SD rats,which then were equally randomized into three groups of A（bleeding wound was treated with montmorillonite powder）,B（blank control）and C（bleeding wound was treated with chitosan hemostatic powder）.Blood from eye socket of 36 rats was collected and put into sodium citrate anticoagulant test tubes,which were equally randomized into three groups and treated as liver bleeding wound meantioned above.The hemostatic time in vivo and coagulation time in vitro test were recorded.One week later,the liver functions and inflammatory factors were measured.Then the pathological change of healed liver tissues was observed by HE staining.Results Compared with group B,the hemostatic time in vivo and coagulation time in vitro test were shorter and the levels of ALT,AST,IL-1,IL-6 and IL-8 were all significantly lower in groups of A and C（P〈0.05）.HE staining indicated that the liver tissues were well-healed in three groups and montmorillonite had no adverse effects on the liver.Conclusion Montmorillonite has effective hemostatic effect on active bleeding wound with good tissue biocompatibility.
作者
王建坤
赵黎黎
刘文婕
何可心
王敏
范志宁
WANG Jiankun;ZHAO Lili;LIU Wenjie(Digestive Endoscopy Center, First A~iliated Hospital, Nanjing Medical University, Nanjing 210029, CHINA)
出处
《江苏医药》
CAS
2018年第5期484-487,F0002,共5页
Jiangsu Medical Journal
基金
国家自然科学基金(81700577)
关键词
蒙脱石
肝脏出血
止血
Montmorillonite
Liver hemorrhage
Hemostasis
