摘要
2016年本实验室从广东省分离到1株禽腺病毒血清4型（FAd V-4）,被命名为HZ-06-01。为研究该毒株的生物学特性,首先将它在鸡肝癌细胞（LMH）上进行传代及蚀斑纯化,然后对该病毒的hexon、ORF19及ORF27基因进行序列分析;结果显示,该毒株与国内代表毒株HB1510、JSJ13的hexon基因的序列相似性为100%,且存在ORF19和ORF27基因的缺失。将HZ-06-01株通过肌肉注射和灌喂两种途径对3周龄SPF鸡攻毒,结果,肌肉注射组死亡率为100%（10/10）,且在病死鸡的多个脏器中均能检测到病毒;灌喂组死亡率为0（0/10）,但攻毒后第2周能检测到抗体;病理组织学观察可见病死鸡肝有明显的核内包涵体、脾淋巴细胞减少等病变。结果表明,HZ-06-01株属于FAd V-4,且注射途径攻毒可以使SPF鸡表现出典型的心包积液综合症。本试验结果为心包积液综合症的诊断及防控提供了基础数据。
One strain of fowl adenovirus serotype 4 named HZ-06-01（FAd V-4）was isolated from Guangdong Province in 2016. To investigate the biological characteristics of this strain,the plaque purification was used to purify the isolated virus on LMH cells.The isolated viral sequences of hexon,ORF19 and ORF27 genes were analyzed,revealing that the identity was 100% between HZ-06-01 and Chinese representative strains HB1510 and JSJ13,and it existed ORF19 and ORF27 gene deletion.Two groups of SPF chickens（three weeks age-old） were challenged by intramuscular and oral administration,respectively.The chickens of intramuscular infection group had higher mortality rate 100%（10/10）,as well as broadly viral detection in multiple organs in dead chickens.However,the chickens of oral administration group also were induced specific antibody on day 14 post-infection and the mortality was 0%（0/10）. Histopathological examination showed that the liver had obvious nucleus inclusion bodies, severe reduction and necrosis of lymphocytes showed in spleen at dead chickens.In conclusion,HZ-06-01 belonged to FAd V-4,and SPF chickens showed obvious HHS by intramuscular administration.The results of this study provide the basic data for the diagnosis and prevention of the disease.
作者
申祖杰
黎世彬
林华源
任行星
洪艳芬
廖佳钰
张建民
廖明
徐成刚
SHEN Zu-jie;LI Shi-bin;LIN Hua-yuan;REN Xing-xing;HONG Yan-fen;LIAO Jia-yu;ZHANG LIAO Ming;XU Cheng-gang(National and Regional Joint Engineering Labaratory for Medicament of Zoonoses Prevention and Control, College of Veterinary Medicine ,South China Agricultural University, Guangzhou 510642, China)
出处
《中国兽医科学》
CAS
CSCD
北大核心
2018年第6期722-728,共7页
Chinese Veterinary Science
基金
国家肉鸡产业技术体系研究项目(CARS-41-G16)
禽流感免疫防控新技术研究项目(2017YFD0500701-4)
广州市科技计划项目(201704020017)
