摘要
建立了高效液相色谱法(HPLC)快速测定印楝素超低容量液剂中有效成分印楝素A的方法.该法采用Agilent C18色谱柱,流动相为乙腈∶5mmol﹒L-1乙酸铵水溶液(40∶60),检测波长218 nm,柱温为35℃.该法的线性范围为10~200μg/m L(R﹥0.998),最低检出限(LOD)为0.5μg/m L,定量限(LOQ)为1.3μg/m L,重复性实验的相对标准偏差(RSD)为3.68%,加标回收率为(102.37±1.78)%.该法适合印楝素超低容量液剂中印楝素A的分析.
The HPLC method was established to quickly determine the azadirachtin A in ultra-low volume. The Agilent C 18 column was used with aeetonitrile-5mmol · L-1 ammonium acetate （40：60） as mobile phase. The detection wavelength was 218nm and the column temperature, 35℃. A good linearity was obtained from 10 to 200 μg/mL （R 〉 0.998）. The limit of detection （LOD） and the limit of quantitation （LOQ） were 0.5 μg/mL and 1.3 μg/mL respectively. The relative standard deviation （RSD） was 3.68% and the recovery rate was 102.37%±SD. The method is suitable for the determination of azadirachtin A.
作者
周靖
翁盼伟
王金林
卢乔墩
崔淑芬
ZHOU Jing;WENG Panwei;WANG Jinlin;LU Qiaodun;CUI Shufen(School of Applied Chemistry and Biotechnology, Shenzhen Polytechnic, Shenzhen, Guangdong 518055, China;Guangdong Lichee ＆ Long, an Pest Control Technological Innovation Center, Shenzhen, Guang,dong 518055, China)
出处
《深圳职业技术学院学报》
CAS
2018年第3期37-40,共4页
Journal of Shenzhen Polytechnic
基金
深圳职业技术学院自然科学基金资助项目(601722k27013)
关键词
印楝素A
超低容量液剂
HPLC
验证
azadirachtin A
ultra-low volume concentrate
HPLC
validation
