摘要
应用免疫磁珠分选结合流式细胞术与透射电镜术对小鼠骨髓造血干细胞进行了分离、纯化及透射电镜观察。结果显示,20只小鼠的骨髓细胞经分离纯化后,共收集到2×105个造血干细胞,纯度达95%以上;其形态类似于小淋巴细胞,呈单核,近圆形,直径约5~7μm,核大细胞质少,染色质电子密度不均,除了线粒体和一些囊泡外,较少见到其他细胞器。经分离纯化的小鼠骨髓造血干细胞纯度好、活率高,可制成透射电镜标本,并能清晰观察其超微结构特征。该研究方法试图找出小鼠骨髓造血干细胞在形态学上的特征,为后续研究提供实验基础,且对探讨造血细胞结构与功能的关系具有一定的应用价值。
The hematopoietic stem cells were isolated and purified from the mice bone marrow using immunomagnetic beads and flow cytometry, and observed by means of transmission electron microscopy. The results showed that 2×105 hematopoietic stem cells were collected from the 20 mice bone marrow, with a purity of more than 95%. They were single nuclear cells, similar to lymphocyte, suborbicular, about 5~7 μm in diameter, large nuclear/cytoplasmic ratios, uneven chromatin electron density. Other organelles were hard to observe, except for some mitochondria and vacuoles. It concluded that the purified mice bone marrow hematopoietic stem cells had high purity and high activity, which could be used as transmission electron microscope specimen and observed its ultrastructure characteristics clearly. This study attempts to find the character of mice bone marrow hematopoietic stem cells in morphology and provide basic data for future research, and to have certain application value in relationship with the structure and function.
作者
张小翠
谷浩
赵犇鹏
符蓉
于卓
Zhang Xiaocui;Gu Hao;Zhao Benpeng;Fu Rong;Yu Zhuo(Core Facility of Basic Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China;Deparment of Pathology and Physiology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China)
出处
《中国细胞生物学学报》
CAS
CSCD
2018年第4期568-572,共5页
Chinese Journal of Cell Biology
基金
上海市自然科学基金(批准号:17ZR1415500)资助的课题~~
