摘要
目的了解HIV阳性的恶性肿瘤患者放化疗期间外周血CD3+、CD3+CD4+、CD3+CD8+比值变化情况并探讨其临床意义。方法收集2010年1月-2016年12月在本院就诊的60例临床资料完整的HIV阳性的恶性肿瘤患者作为实验组,收集同期60名健康对照组及60名HIV阴性的肿瘤患者作为对照组,采用多参数流式细胞术(FCM)检测两组研究对象外周血CD3+、CD3+CD4+、CD3+CD8+比值变化情况。结果与健康对照组及HIV阴性的肿瘤患者比较,HIV阳性的恶性肿瘤患者外周血CD3+,CD3+CD4+细胞比例及CD3+CD4+/CD3+CD8+比值明显低于健康对照组及HIV阴性的肿瘤患者,CD3+CD8+细胞比例明显高于健康对照组及HIV阴性的肿瘤患者(P<0.01)。放化疗后患者外周血CD3+、CD3+CD4+细胞比例及CD3+CD4+/CD3+CD8+比值明显低于其放化疗前(P<0.01),CD3+CD8+细胞比例明显高于其放化疗前,以上差异均有统计学意义(P均<0.01)。恶性肿瘤患者放化疗后外周血CD3+CD4+/CD3+CD8+比值与其性别、KPS评分、病理类型、有无淋巴结转移均无相关性(P均>0.05),而与其年龄、肿瘤浸润深度及临床分期相关(P均<0.05)。结论 HIV阳性的恶性肿瘤患者细胞免疫功能紊乱,检测其T细胞亚群水平对疾病诊断及预后判断具有积极意义。
Objective To explore the changes of CD3＋, CD3＋CD4＋, CD3＋CD8＋ ratio in patients with malignant tumor who were clinical diagnosed with HIV positive, and to analyze its clinical significance.Methods Sixty cases of HIV positive patients with complete clinical tumor data were collected in our hospital from January 2010 to December 2016 as the experimental group, during the same period, sixty HIV negative tumor patients were presented as control group, and other sixty were collected as healthy control group.Multi parameter flow cytometry（FCM）was used to detect the change of CD3＋,CD3＋CD4＋, CD3＋CD8＋ ratio in the two groups.Results Compared with healthy control group and HIV negative tumor patients, peripheral blood CD3＋,CD3＋CD4＋and CD3＋CD4＋/CD3＋CD8＋ cell ratio were significantly lower in HIV positive patients（P〈0.01）, and the ratio of CD3＋ CD8＋ was significantly higher than the healthy control group and HIV negative tumor patients（P〈0.01）. Compared with before chemotherapy and radiotherapy, the proportion of CD3＋, CD3＋CD4＋ and CD3＋CD4＋/CD3＋CD8＋ ratio was significantly lower in after chemotherapy and radiotherapy（P〈0.01）,but the ratio were of CD3＋CD8＋ was significantly higher than that before chemotherapy and radiotherapy（P〈0.01）; patients with malignant tumor after radiotherapy and chemotherapy,CD3＋CD4＋/CD3＋CD8＋ ratio not associated with gender,KPS score, pathological type, lymph node metastasis（P〉0.05）, but associated with patients, age, depth of tumor invasion and clinical staging（P〈0.05）.Conclusion HIV positive tumor patients with cellular immune dysfunction, observation and detection of the level of T cell subsets are significant in diagnosis and prognosis of the disease.
作者
徐晨
蒋安
杨翰君
XU Chen;JIANG An;YANG Hanjun(Western Hospital of Chengdu ,Sichuan Province, Chengdu 610017, China;Department of Dermatology, Southwest Hospital, Third Military Medical University, Chongqing 400032, China;Department of Dermatology, West China Fourth Hospital ,Sichuan University, Chengdu 610000, China)
出处
《中国皮肤性病学杂志》
CAS
CSCD
北大核心
2018年第6期658-661,共4页
The Chinese Journal of Dermatovenereology
关键词
HIV阳性
恶性肿瘤
放疗
化疗
T细胞亚群
HIV positive
Malignant tumor
Radiotherapy
Chemotherapy
T lymphocyte subsets
