摘要
以天麻为研究对象,采用响应面法优化天麻蛋白的提取条件。在单因素水平测定的基础上,选取提取温度、提取时间、盐含量作为影响因素,应用Box-Behnken中心组合进行3因素3水平的试验设计,以对白色念珠菌的抑菌率作为响应值,进行响应面分析。将最佳工艺条件下得到的天麻蛋白浓缩液梯度稀释,进行量效和时效曲线的测定。结果表明,天麻蛋白的最佳提取条件为:提取温度77℃,提取时间4 h,盐含量0.8%,在此工艺条件下进行3次验证试验,对白色念珠菌的平均抑菌率为88.23%,与预测值(89.08%)接近。天麻蛋白质量浓度为1 g/m L,蛋白含量0.596 g/m L,与白色念珠菌作用5 min时,抑菌率在90%以上。响应面法优化的天麻蛋白浓缩液对白色念珠菌、绿脓杆菌、大肠杆菌、金黄色葡萄球菌具有很强的抑菌活性,抑菌圈直径分别为20.89,22.02,22.71,16.73 mm。
Gastrodia elata as the research object. Response surface analysis methodology（RSM） be used for optimizing the Extraction of Gastrodia elata protein. Based on single-factor experiment, three independable variables, namely extraction temperature, extraction time, salt concentration, were selected as casual factors, and the experiment were arranged according to Box-Behnken central composite experiment design. Response surface analysis were applied to determine the effect of central composite experiment design and the bacteriostatic rate against candida albicans was used as the responsive values. Gastrodia elata protein concentration was gradiented dilution, and determine its Dose-and time-dependent curves. The results showed that the optimum conditions were as follows： extraction temperature 77 ℃, extraction time 4 h, salt concentration 0.8%, under these conditions, the average bacteriostatic rate verified by three experiments was 88.23%, closing to the predicted value（89.08%）. The mass concentration of extraction is 1 g/mL, protein concentration is 0.596 g/mL, antibacterial rate is more than 90% against candida albicans 5 min. At the same time, the response surface optimization of the extraction of Gastrodia elata protein have an significant bacteriostatic action on candida albicans, pseudomonas aeruginosa, escherichia coli, staphylococcus aureus. Bacteriostatic ring diameter is that 20.89,22.02, 22.71 mm and 16.73 mm.
出处
《中国食品学报》
EI
CAS
CSCD
北大核心
2018年第4期176-182,共7页
Journal of Chinese Institute Of Food Science and Technology
基金
国家自然科学基金项目(814029055)
关键词
天麻
响应面分析
量效-时效曲线
抑菌活性
Gastrodia elata
response surface analysis
dose-and time-dependent curves
antibacterial activity
