摘要
目的 明确受体相互作用蛋白140 （RIP140）在姜黄素抑制胰岛β细胞株MIN6细胞凋亡中的作用.方法 将MIN6细胞分为对照组、高糖高脂处理组、姜黄素干预组、O-RIP140-MIN6组（过表达RIP140细胞株）、GFP-MIN6组（对照细胞株）.除对照组外,其余各组细胞给予25 mmol/L葡萄糖＋ 500μmol/L棕榈酸处理24 h.姜黄素干预组与O-RIP140-MIN6组、GFP-MIN6组均在25 mmol/L葡萄糖＋500 μmol/L棕榈酸处理24h前给予20 μmol/L姜黄素预处理2h.流式细胞仪检测细胞凋亡率、Western印迹检测B细胞淋巴瘤-2（Bcl-2）、细胞外信号调节激酶（ERK）及其磷酸化水平.结果 与对照组相比,高糖高脂处理组RIP140蛋白表达、细胞凋亡率及ERK磷酸化水平均明显增加、Bcl-2表达下降（F=22.42～111.43,P均＜0.01）;与高糖高脂处理组相比,姜黄素干预组细胞凋亡率及RIP140蛋白表达均明显下降、ERK磷酸化水平明显降低,Bcl-2表达升高（F=22.42～111.43,P均＜0.01）;与GFP-MIN6组相比,O-RIP140-MIN6组细胞凋亡率明显升高（t=7.16,P＜0.01）、ERK的磷酸化水平升高（t=8.37,P＜0.01）,Bcl-2表达降低（t=5.88,P＜0.05）.结论 姜黄素通过下调RIP140的表达来抑制高糖高脂诱导的胰岛β细胞凋亡.
Objective To investigate the role of receptor interaction protein 140 （RIP140） in the inhibition of curcumin on apoptosis in MIN6 cells.Methods MIN6 cells were divided into five groups：control group,high glucose and lipid group,curcumin group,O-RIP140-MIN6 group （MIN6 cells with overexpressed RIP140） and GFP-MIN6 group （empty vector group）.Except for control group,all other groups were treated with 25 mmol/L glucose and 500 μmol/L palmitic acid for 24 hours.Cells in curcumin group,O-RIP140-MIN6 group and GFP-MIN6 group were pretreated with 20 μmol/L curcumin for 2 hours before the treatment of 25 mmol/L glucose and 500 μmol/L palmitic acid for 24 hours.Apoptosis rate was detected by flow cytometry,the expression of B-cell lymphoma 2 （Bcl-2）,extracellular regulated protein kinases （ERK） and phosphated-ERK were tested by Western blotting.Results Compared with control group,the expression of RIP140 protein,apoptosis rate and the level of phosphated-ERK were significantly increased whereas the expression of Bcl-2 was decreased in high glucose and lipid group （F =22.42-111.43,all P 〈 0.01）.However,compared with high glucose and lipid group,the apoptosis rate and the expression of RIP140 and phosphated-ERK were inhibited whereas the expression of Bcl-2 was increased in curcumin group（F=22.42-111.43,all P〈0.01）.Compared with GFP-MIN6 group,the apoptosis rate （t =7.16,P 〈 0.01） and the level of phosphated-ERK （t =8.37,P 〈 0.01） were increased and the expression of Bcl-2（t =5.88,P 〈0.05） was decreased in O-RIP140-MIN6 group.Conclusion Curcumin inhibits glucolipotoxicity-induced apoptosis in islet β cells via the downregulation of RIP140 expression.
作者
薛君力
代喆
曾姣娥
徐焱成
Xue Junli;Dai Zhe;Zeng Jiao＇e;Xu Yancheng(Department of Endocrinology, Jingzhou Central Hospital, Jingzhou 434020, Chin)
出处
《国际内分泌代谢杂志》
2018年第3期154-157,162,共5页
International Journal of Endocrinology and Metabolism
基金
国家自然科学基金（81170769,81370872）
湖北省卫生计生科研基金（WJ2016-YZ-06）
