摘要
目的:优化松针总黄酮提取工艺,为合理利用东北松针资源提供理论依据。方法:以黑龙江省小兴安岭红松松针为原料,在单因素实验基础上,结合Box-Benhnken中心组合原理及响应面优化实验条件。结果:最优提取工艺参数:料液比为1∶65 g/mL,超声波功率比为70%,超声总时间为46 min,乙醇体积分数为55%,工作与间歇时间比为3/2(s/s)。该条件下松针总黄酮得率约为4.88%,为超声波细胞粉碎法提取松针黄酮的最佳工艺条件。结论:超声波细胞粉碎法更省时,可显著提高松针总黄酮得率,该方法为东北红松松针黄酮的提取提供了理论依据。
Objective：optimization of extraction process of total flavonoids in pine needles to provide theoretical basis for rational utilization of pine needles resources in Northeast China. Methods ：using pine needles of Pinus koraiensis needle in Xiaoxing＇anling of Heilongjiang province as raw materials,on the basis of single factor experiment,combined the principle of Box-Benhnken center combination and response surface optimization experimental conditions. Results ：optimum extraction process parameters ：the solid to liquid ratio is 1 ∶ 65 g/mL;the ultrasonic power ratio is 70%;the total time of ultrasound was 46 min;the ethanol volume fraction was 55%;the ratio of work to intermittent time is 3/2 s/s. Under these conditions,the yield of total flavonoids in pine needles was about 4.88%,thus,were the best conditions for extracting flavonoids from pine needles by ultrasonic cell pulverization. Conclusion ：ultrasonic cell crushing method saves time and significantly improves the efficiency of flavonoids extraction from pine needles. The method provides a theoretical basis for the extraction of flavonoids from pine needles of northeast pine.
作者
刘宁
王冉
李健
刘涛
黎晨晨
LIU Ning, WANG Ran, LI Jian, LIU Tao, LI Chen-chen(College of Food Engineering, Harbin University of Commerce, Harbin 15007)
出处
《中国食品添加剂》
CAS
2018年第4期118-127,共10页
China Food Additives
基金
黑龙江省教育厅科学技术面上项目(12541199)
2016年哈尔滨商业大学青年创新人才支持项目(编号2016QN068)
关键词
细胞破碎
提取工艺
总黄酮
cell disruption
total flavonoids
extraction technology
