摘要
目的 探讨粒细胞集落刺激因子（G-CSF）对新生大鼠缺氧缺血性脑损伤（HIBD）后神经细胞凋亡的影响及哺乳动物雷帕霉素靶蛋白（mTOR）/p70S6K信号通路在其中发挥的作用. 方法 90只7d龄新生SD大鼠按随机数字表法分为假手术组、模型组、G-CSF组、雷帕霉素组及对照组,每组18只.后4组采用改良Rice-Vannucci法制备成HIBD模型,其中雷帕霉素组、对照组于HIBD前1h分别腹腔注射雷帕霉素250μg/kg或等体积乙醇;G-CSF组、雷帕霉素组及对照组于HIBD后1h腹腔注射G-CSF 50 μg/kg,假手术组、模型组予等体积生理盐水腹腔注射.HIBD后48h,采用2,3,5-三苯基氯化四氮唑（TTC）染色检测脑梗死面积并计算梗死面积比,Nissl染色评估海马CA1区及皮质神经细胞情况,TUNEL法检测海马CA1区及皮质神经细胞凋亡情况,Westernblotting检测脑组织中抗凋亡因子B淋巴细胞瘤-2基因（Bcl-2）及促凋亡因子Caspase-3活化片段（CC3）、Bcl-2相关X蛋白（Bax）,mTOR、磷酸化（p）-mTOR及其下游p70S6K、p-p70S6K蛋白的表达. 结果 与模型组相比,G-CSF组和对照组梗死面积比明显减少,海马CA1区及皮质Nissl染色阳性细胞数明显增多、神经细胞凋亡指数明显降低,脑组织中CC3、Bax蛋白表达水平明显降低,Bcl-2蛋白表达水平明显升高,p-mTOR/mTOR、p-p70S6K/p70S6K比值明显升高,差异均有统计学意义（P＜0.05）.与G-CSF组和对照组相比,雷帕霉素组梗死面积比明显增加,海马CA1区及皮质Nissl染色阳性细胞数明显减少、神经细胞凋亡指数明显增加,脑组织中CC3、Bax蛋白表达水平明显升高,Bcl-2蛋白表达水平明显降低,p-mTOR/mTOR、p-p70S6K/p70S6K比值明显降低,差异均有统计学意义（P＜0.05）. 结论 G-CSF可能通过上调mTOR/p70S6K信号通路抑制HIBD后神经细胞凋亡.
Objective To investigate the effects of granulocyte-colony stimulating factor （G-CSF） on neuronal apoptosis after hypoxia-ischemia brain damage （HIBD） and the possible role of the mTOR/p70S6K signaling pathway in neonatal rats.Methods Ninety seven-day-old Sprague-Dawley rats were assigned into 5 equal groups （n=18）：sham group,HIBD group,G-CSF group,rapamycin group and ethanol group by random number table method.Pups were subjected to unilateral carotid artery ligation followed by 2hrs hypoxia or sham surgery.HIBD animals received normal saline,G-CSF （50 μg/kg）,G-CSF combined with rapamycin （250 μg/kg） or ethanol （vehicle for rapamycin）.Pups were euthanized 48hrs post-HIBD to quantify the percentage of brain infraction area.The pathomorphologic changes in the hippocampal CA 1 area and cortex were observed by Nissl staining.Neuronal cell death was determined using terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling （TUNEL）.mTOR,activated mTOR （p-mTOR）,p70S6K,activated p70S6K （p-p70S6K）,Cleaved Caspase-3 （CC3）,Bax,and Bcl-2 were quantified using Western blot analysis.Results G-CSF treatment resulted in significantly reduced percentage of brain infraction area （P＜0.05） and neuronal apoptosis in the hippocampal CA1 area and cortex （P＜0.05） after HIBD in neonatal rats.However,rapamycin administration reversed the neuroprotective effect of G-CSF.G-CSF administration ameliorated the pathomorphologic damage in the ipsilateral hemisphere.Compared with the HIBD group,the Nissl stained neurons significantly increased in the G-CSF group （P＜0.05）.Furthermore,G-CSF increased the expression ofp-mTOR,p-p70S6K and Bcl-2 but decreased the expression levels of CC3 and Bax in the ipsilateral hemisphere,which were all significantly reversed by rapamycin （P＜0.05）.Conclusion G-CSF may attenuate caspase activation and reduce neuronal apoptosis by up-regulating the activity of mTOR/p70S6K signaling pathway after experimental HIBD in rat pups.
作者
陈璐
舒斯云
吴政彦
马林
杜江
Dumbuya John Sieh
罗薇
李菲
王斌
Chen Lu;Shu Siyun;Wu Zhengyan;Ma Lin;Du Jiang;Dumbuya John Sieh;Luo Wei;Li Fei;Wang Bin(Department of Pediatrics, Zhujiang Hospital, Southern Medical University, Gnangzhou Key Laboratory of Inflammation and Immune Diseases, Guangzhou 510282, China;Department of Biomedical Science, Charles E. Schmidt College of Medicine, Florida Atlantic University, 33431 Boca Raton, USA;Department of Radiotherapy, Chinese PLA General Hospital, 100853 Beijing, China)
出处
《中华神经医学杂志》
CAS
CSCD
北大核心
2018年第5期450-456,共7页
Chinese Journal of Neuromedicine
基金
国家自然科学基金（81371514）
