摘要
为了研究不同菌源降解乙酸的产气效果和代谢途径,选用煤层水和污泥2种产甲烷菌菌源进行了为期56 d的生物成气试验,分析了2种菌源降解乙酸的最佳浓度、抑制浓度及产气规律,并分析了2种菌源降解乙酸的代谢途径。结果表明,煤层水菌源和污泥菌源都能降解乙酸产气,并且在乙酸浓度为0.5‰时产气最佳,但是当乙酸浓度大于5‰时,产甲烷过程受到抑制;2种菌源降解乙酸的产气过程都经历了产气量缓慢增加阶段—急剧增加阶段—相对稳定阶段3个阶段,而且大量产气基本在21~42 d时。通过分析产气中的各组分含量,初步判断实验中煤层水菌源生成甲烷的代谢途径主要为二氧化碳还原,污泥菌源生成甲烷的代谢途径主要为乙酸发酵。
In order to study the gas effects and metabolic pathways of different bacteria sources degrade acetic acid,selecting coalbed water and sludge methanogenic bacteria source,biogas test of 56 days was conducted,the optimal concentration of two strains of acetic acid degradation and inhibition concentration and gas production rules were analysed,and the metabolic pathways for the degradation of acetic acid by two kinds of bacteria sources were revealed.The result showed that the coalbed water bacteria source and sludge source can degrade acetic acid to product gas,and gas production is best when the concentration of acetic acid is 0.5 per thousand,but when the concentration of acetic acid is more than 5 per thousand,methane production was inhibited;the gas production process in two strains bacteria sources have experienced three stages：slow increase stage-sharp increase stage-relatively stable stage,and a large number of gas production are basically in 21-42 d;found by analysis in the gas content,it is preliminarily determined that the main metabolic pathway of methane production by the coalbed water source is the reduction of carbon dioxide,the main metabolic pathway of methane production by sludge bacteria source is acetic acid fermentation.
作者
王美林
Wang Meilin(State Key Laboratory of Coal and CBM Co-Mining,Jincheng 048012,China;Yian Lanyan Coal and CBM Co-Mining Technology Co.,Ltd.,Jincheng 048012,China)
出处
《能源与环保》
2018年第5期84-87,90,共5页
CHINA ENERGY AND ENVIRONMENTAL PROTECTION
基金
山西省煤基重点科技攻关资助项目(MQ2014-03)
山西省煤层气联合研究基金资助项目(2013012016
2015012001)
关键词
产甲烷菌
生物成气
乙酸
成气效果
代谢途径
methanogenic bacteria
biogenic gas
acetic acid
gas production effect
metabolic pathway
