摘要
目的探讨蛇床子素对睡眠剥夺大鼠记忆功能的影响。方法将48只雄性大鼠随机分为正常对照组(NC组)、大平台对照组(TC组)、睡眠剥夺组(M组)和蛇床子素组(Ost组)。采用改良多平台法建立大鼠睡眠剥夺模型、Morris水迷宫检测大鼠学习记忆能力、HE染色法观察大鼠海马区神经元形态的改变,并检测海马组织与血清中丙二醛(MDA)、超氧化物歧化酶(SOD)以及海马组织NO的含量。结果与NC组及TC组相比,M组大鼠逃避潜伏期显著升高而穿越平台次数显著减少,海马组织与血清MDA含量和海马组织SOD活性、NO含量均升高;补充蛇床子素后,大鼠逃避潜伏期显著缩短,平台穿越次数增加,海马组织与血清MDA含量降低,同时海马组织SOD活力恢复到正常水平,但海马组织NO含量未降低。结论蛇床子素可通过抗氧化应激,减轻海马组织神经细胞的损害对睡眠剥夺大鼠的记忆功能产生明显的保护作用。
Objective To investigate the effect of Osthole on memory function of sleep deprivation（SD）mice. Methods Forty-eight male mice were randomly divided into 4 groups;normal control group（NC group）,large platform control group（TC group）,sleep deprivation group（M group）and Osthole group（Ost group）. Themodel of SD in mice was estabished by using improved multi platform method. The ability of learning and memorywas tested by using Morris water maze test and pathological changes of hippocampal neurons in mice were observedby HE staining. The serum,hippcampus malondialdehyde（MDA）contents and superoxide dismutase（SOD）activity,so as the hippocampus No content,were detected. Results Compared with NC group and TC group,the escape la-tency of M group increased significiantly and the number of crossing platform decreased significantly. There were in-creased levels hippocampus tissue,serum MDA level,hippocampal SOD activity and NO content. After supplemen-tation of Osthole,the escape latency significantly shortened in mice. The number of crossing platform was increasedwhile the contents of MDA both in hippocampus and serum were decreased,and the SOD activity in hippocampus re-turned to normal. However,the level of NO in hippocampus was not decreased. Conclusion Osthole can protect thememory function of SD mice by reducing the the damage of hippocampal neurons through antioxidant stress.
作者
杜展鑫
唐珮瑜
谢炜基
潘晓佳
罗伟聪
陈棋棋
欧超然
梁建芬
朱晓琴
DU Zhanxin;TANG Peiyu;XIE Weiji;PAN Xiaojia;LUO Weicong;CHEN Qiqi;OU Chaoran;LIANG Jianfen;ZHU Xiaoqin(Guangzhou Medical University,Guangzhou 511436,China)
出处
《实用医学杂志》
CAS
北大核心
2018年第10期1633-1635,1639,共4页
The Journal of Practical Medicine
基金
2017年度国家级大学生创新创业训练计划项目(编号:201710570005)
2017年度广东大学生科研创新培育专项资金("攀登计划"专项资金项目(编号:pdjh2017b0417)
2017-2018年度广州医科大学大学生科技创新项目(编号:2016A13)
2018年度广州医科大学大学生实验室开放项目(编号:20182709)
关键词
蛇床子素
睡眠剥夺
学习记忆
抗氧化
osthole
sleep deprivation
learning memory
antioxidant
