摘要
目的:探讨脂多糖(lipopolysaccharide,LPS)对人牙髓干细胞(human Dental pulp Stem cells,h DPSCs)增殖及骨向分化的作用关系。方法:将第3代h DPSCs体外常规培养后,采用MTT法分别检测不同浓度LPS(0、1、10、100、1000 ng/mL)作用h DPSCs 72 h后细胞增殖情况,筛选出促进h DPSCs增殖的LPS最佳浓度。通过Western Blot法检测该浓度作用下h DPSCs中各矿化相关蛋白:碱性磷酸酶(ALP)、骨钙素(OCN)、骨唾液酸蛋白(BSP)及牙本质涎磷蛋白(DSPP)的表达水平。结果:与对照组比较,1 ng/mL及10 ng/mL LPS干预组能明显促进h DPSCs的增殖(P<0.05),其中以10 ng/mL LPS作用更显著。Western Blot检测结果表明,10 ng/mL LPS可明显上调h DPSCs中各矿化相关蛋白的表达水平。结论:低浓度LPS可促进h DPSCs的增殖和骨向分化。
Objective： To explore the effect of lipopolysaccharide（ LPS） in the regulation of proliferation and osteogenic differentiation of human dental pulp stem cells. Methods： The third generation of human dental pulp stem cells were cultured in vitro and treated with LPS for 72 hours at different concentrations of LPS（ 0,1,10,100 and 1000 ng/mL） respectively. MTT assay was employed to evaluate the optimum concentration of LPS for promotion cell proliferati on. The protein expression level of ALP,OCN,BSP and DSPP in h DPSCs treated with low concentration of LPS were examined respectively by Western Blot. Results： The proliferation of h DPSCs were obviously induced by low concentration of LPS（ 1 ng/mL and 10 ng/mL）,and 10 ng/mL LPS was better to promote cell proliferation. Western Blot results showed that 10 ng/mL LPS up-regulated expression of osteogenic differentiation related proteins. Conclusion： LPS can enhance proliferation and osteogenic differentiation of h DPSCs at low concentration.
作者
施俊
宋雨红
贺维
冷卫东
彭兴春
SHI dun1, SONG Yu- hong1,HE Wei1,LENG Wei-dong1,PENG Xing- chun2(1.Department of Stomatology , Taihe Hospital, Hubei University of Medicine, Hubei Shiyan 442000, China ;2. School of Basic Medical Sciences ,Hubei University of Medicine, Hubei Shiyan 442000, Chin)
出处
《临床口腔医学杂志》
2018年第4期209-212,共4页
Journal of Clinical Stomatology
基金
湖北医药学院研究生启动金计划项目(2011QDZR-21)
关键词
脂多糖(LPS)
人牙髓干细胞
增殖
分化
Lipopolysaccharide（LPS）
Dental pulp stem cell
Proliferation
Differentiation
