摘要
【目的】比较七舍古茶等19份茶树资源的咖啡碱合成酶活性,为选育低咖啡碱茶树品种奠定基础。【方法】以19份茶树资源的鲜叶为试材,粗提咖啡碱合成酶,体外进行咖啡碱合成的酶促反应并用高效液相检测各反应体系的咖啡碱含量,比较不同茶树嫩叶的咖啡碱合成酶的活性。【结果】七舍古茶的咖啡碱合成酶活性最高,为0.386 U/mL;其次是保靖黄金茶、黄金叶,分别为0.661和0.090 U/mL;盘县古茶的咖啡碱合成酶活性最低,为0.038 U/mL;龙井长叶等其余15份茶树资源的咖啡碱合成酶活性差异不大,在0.043~0.07 U/mL。【结论】盘县古茶树可以作为低咖啡碱品种选育的基础材料。
【Objective】The study aimed to lay the foundation for breeding of tea varieties with asynthase activity of 19 tea germplasm resources. 【Method】The caffeine content in fresh leaves of 19 tea germplasm resources was detected by HPLCafter in-vitro enzymatic reaction end of caffeine synthase to compare caffeine synthase activity of 19 tea germplasm resources.【Result】The caffeine synthase activity of Qishedachashu, Baojinghuangjincha and Huangjinye was 0.386, 0.161 and 0.090 U/mL respec the caffeine synthase activity of Panxiandachashu was 0.038 U/mL only. There was no obvious difference in caffeine synthase activity among other 15 tea germplasm resources and their caffeine synthase activity was 0.043-0.07 U/mL. 【Conclusion】Panxiandachashu variety can beused as a basic material for breeding of tea varieties with a low caffeine content.
作者
段学艺
高秀兵
曹雨
赵华富
张圆
何萍
胡华健
DUAN Xueyi;GAO Xiubing;CAO Yu;ZHAO Huafu;ZHANG Yuan;HE Ping;HU Huajian(Guizhou Tea Research Institute,Guizhou Guiyang 550006,Chin)
出处
《西南农业学报》
CSCD
北大核心
2018年第5期907-910,共4页
Southwest China Journal of Agricultural Sciences
基金
贵州省科学技术基金项目"低咖啡因大树茶单株资源种质评价与遗传多样性分析"[黔科合J字LKN(2013)16]
关键词
茶树资源
咖啡碱
咖啡碱合成酶
酶活性
Tea germplasm resource
Caffeine
Caffeine synthase
Activity
