摘要
目的调查成都地区中老年人肠道菌群组成与其血脂、血糖代谢间的可能关系。方法于2016年11月在成都市纳入51例中老年人,收集其血液及粪便样本,测定其血糖、血脂,使用Illumina高通量测序技术对粪便样本中肠道菌群的丰度、多样性及菌种组成进行详细分析。结果受试中老年人粪便细菌主要为厚壁菌门、拟杆菌门、变形菌门、梭杆菌门、放线菌门、疣微菌门。高血糖受试中老年人的粪便细菌多样性(Shannon指数)低于血糖正常的受试中老年人(P<0.05),但是Shannon指数和血脂无明显关系。在粪便细菌门水平上:变形菌门、拟杆菌门分别在高血糖的中年、老年女性受试者粪便中的比例高于血糖正常受试中年、老年女性(P<0.05)。在粪便细菌属水平上:粪杆菌在高血糖受试中老年人粪便中的比例降低(P<0.05),且其丰度与血糖水平呈负相关(r=-0.278,P=0.048);普氏菌属、帕拉普氏菌属在高血脂组的比例降低(P<0.05),且均与血脂水平呈负相关(r=-0.357,P=0.10;r=-0.365,P=0.008)。结论不同血糖、血脂水平的中老年人其肠道菌群的构成和数量存在显著差异,表明肠道菌群与中老年人血糖、血脂代谢可能有着密切的关系,在中老年人血糖、血脂相关疾病的预防及治疗中可能有重要意义。
Objective To investigate the possible relationship between the gut microbiota and hyperlipidemia,hyperglycemia in middle-aged and elderly people in Chengdu.Methods 51 of middle-aged and elderly people participated in this study.The concentration of blood glucose and lipid,and the other physiological parameters of the subjects were analyzed,and their fecal microbiota was also profiled by Illumina high-throughput sequencing technique.Results The fecal microbiota in the middle-aged and elderly were mainly Firmicutes,Bacteroidetes,Proteobacteria,Fusobacteria,Actinobacteria,Verrucomicrobia.The diversity offecalmicrobiota was significantly lower in the subjects with higher blood glucose（P〈0.05）,while it did not exhibited relationship with changes in blood lipids.At the bacterial phyla：Proteobacteria and Bacteroidetes were respectively significantly higher in middle-aged and elderly women with higher blood glucose（P〈0.05）.At the bacterial genus：Faecalibacterium was significantly lower in the hyperglycemic subjects（P〈0.05）,its abundance and blood glucose levels were negatively correlated（r=-0.278,P=0.048）;Prevotellaand Paraprevotella were significantly lower in the high blood lipids group（P〈0.05）,and both of them were negatively correlated with blood lipid levels（r=-0.357,P=0.10;r=-0.365,P=0.008）.Conclusion The fecal microbiota in the tested middle-aged and elderly subjects varied with blood glucose and blood lipid concentration.Intestinal microbes might posses close relationship with blood glucose and lipid metabolism in middle-aged and elderly people.
作者
王柯
万群
葛林
于晓红
李鸣
郭云
胡敏
马新颖
崔文静
何方
WANG Ke;WAN Qun;GE Lin;YU Xiao-hong;LI Ming;GUO Yun;HU Min;MA Xin-ying;CUI Wen-jing;HE Fang(Department of Nutrition, Food Safety and Toxicology, West China School of Public Health, Sichuan University, Chengdu 610041, China;Huayang Community Health Service Center, Chengdu Tianfu New Area, Chengdu 610213, China;Hebei Inatural Biotech Co. Ltd. , Shijiazhuang 050800, China)
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2018年第3期408-413,共6页
Journal of Sichuan University(Medical Sciences)
基金
河北省科技计划项目(No.14392801D)资助
关键词
高通量测序
肠道菌群
血糖
血脂
High-throughput sequencing technology
Gut microbiota
Blood glucose
Blood lipid
