摘要
目的研究通咽喷雾剂对线栓法短暂性大脑中动脉阻塞(transient middle cerebral artery occlusion,t MCAO)模型大鼠吞咽功能的改善并初步探讨其作用机理。方法雄性SD大鼠随机分为正常组、假手术组、模型组和药物组,后两组采用线栓法短暂性阻塞大脑中动脉90分钟造模,药物组给予通咽喷雾剂治疗14天。于造模后第7天、第14天记录各组大鼠在相同时间内由蒸馏水引发的吞咽次数和吞咽反应时间;ELISA法检测各组血清P物质(substance P,SP)含量;免疫组化法检测各组延髓孤束核内P物质(substance P,SP)、5-羟色胺(serotonin,5-HT)含量。结果造模后第14天模型组大鼠吞咽次数减少(P<0.01)、吞咽反应时间延迟(P<0.05),药物组吞咽次数增加(P<0.05);模型组血清SP含量降低(P<0.01)、延髓孤束核内SP及5-HT阳性神经纤维含量减少(P<0.05,P<0.01);药物组血清SP含量增加(P<0.05),延髓孤束核内SP及5-HT阳性神经纤维含量增加(P<0.05,P<0.01)。结论通咽喷雾剂可改善t MCAO大鼠的吞咽功能,其作用机制可能与增加血清SP及孤束核内SP与5-HT阳性神经纤维含量,激活辣椒素敏感类神经有关。
Objective To study the improvement of swallowing function of transient middle cerebral artery occlusion（ t MCAO） rats treated with Tongyan spray and to preliminarily explore its mechanism.Methods Male SD rats were randomly divided into control group,sham operation group,model group and drug group. The latter two groups were conducted with 90 min suture method to induce transient middle cerebral artery occlusion. The drug group were treated with Tongyan spray for 14 days. The number of swallowing caused by distilled water and swallowing latency time of each group were recorded on the 7 thday and 14 thday.The level of serum substance P（ SP） in each group were detected by ELISA,and the contents of SP and serotonin（ 5-HT） in the nucleus tractus solitarius（ NTS） of medulla oblongata were evaluated by immunohistochemistry. Results On the fourteenth day after modeling,the number of swallowing in the model group was reduced（ P 〈 0. 01）,the time of swallowing reaction was delayed（ P 〈 0. 05）,and the number of swallowingin the drug group was increased（ P 〈 0. 05）. The content of serum SP in the model group was decreased（ P〈 0. 01）,the content of SP and 5-HT positive nerve fibers in the nucleus of the medulla were decreased（ P〈 0. 05,P 〈 0. 01）. The content of SP in the serum of the drug group was increased（ P 〈 0. 05）,and the content of SP and 5-HT positive nerve fibers in the nucleus of the medullary solitary tract was increased（ P 〈0. 05,P 〈 0. 01）. Conclusion Tongyan spray can improve the swallowing function of t MCAO rats. Its mechanism may be related to the increase of serum SP and the content of SP and 5-HT positive nerve fibers in the nucleus of the solitary tract,and the activation of capsaicin sensitive nerve.
作者
李彩彩
简郭血骄
杨向竹
郭健
冯学功
马鑫
高誉珊
LI Caicai;JIAN Guo xue-jiao;YANG Xiangzhu(School of Traditional Chinese Medicine, Beijing University of ChineseMedicine,Beijing 100029,China)
出处
《环球中医药》
CAS
2018年第5期676-680,共5页
Global Traditional Chinese Medicine
基金
国家自然科学基金(81473623)
