摘要
研究建立了肉制品中亚麻酸的气相色谱检测方法,样品经氢氧化钾-甲醇溶液甲酯化后用GC法分析,通过比对亚麻酸甲酯标准品气相色谱,其保留时间为26.212 min,采用外标法进行定量。结果表明,建立的牛肉中亚麻酸的分析方法在0.01~10.00 mg/m L线性关系良好,相关系数为0.999 95,日内、日间精密度范围分别为0.73%~1.08%和0.75%~1.01%,均小于15%,回收率为83.3%~95.79%,说明该方法灵敏度好、准确度高、线性好,可用于测定牛肉中的亚麻酸含量。
Linolenate was extracted with Isooctane from beefs,and methylified with KOH-CH3 OH solution. Then content of methyl linolenate,which could be translated to the content of linolenate,was quantified with gas chromatographic. The retention time for methyl linolenate was 26.212 min. This method was appicable in a linear range of 0.01-10.00 mg/m L with the correlation coefficient at 0.999 95. The relative standard deviation（RSD） of intra-day and inter-day were 0.73%-1.08% and 0.75%-1.01%,which were less than 15%. The recovery rate was 83.3%-95.79%. The method gave high sensitivity,wide linearity,and specificity,which is suitable for determination of linolenate in beef.
作者
席斌
陈效威
高雅琴
李维红
郭天芬
杜天庆
杨晓玲
XI Bin;CHEN Xiao-wei;GAO Ya-qin;LI Wei-hong;GUO Tian-fen;DU Tian-qing;YANG Xiao-ling(Lanzhou Institute of Animal Husban＆7 and Pharmaceutical Science, Chinese Academy of Agricultural Sciences/Quality Safety Risk Assessment of Animal Products （Lanzhou）,Minist~7 of Agriculture/Quality Supervising,Inspection and Testing Center for Animal Fiber,Fur Leather and Products,Ministry of Agricuhure,Lanzhou 730050,Chin)
出处
《湖北农业科学》
2018年第9期97-99,103,共4页
Hubei Agricultural Sciences
基金
国家农产品质量安全风险评估项目(GJFP201701514)
