摘要
以寒兰的新鲜营养根为材料,采用徒手切片法和苯胺蓝染色压片法,研究寒兰菌根的显微结构和菌根真菌的侵染方式,并采用常规分离法,对其内生真菌进行分离和初步鉴定。结果表明:寒兰(Cymbidium kanran)营养根具有典型的兰科植物菌根构造,菌根真菌通过破坏根被细胞进入根被,侵染皮层组织,在皮层薄壁细胞内形成菌丝团;从菌根中共分离到36个真菌菌株,包括帚霉属、丝核菌属、丛梗孢属、色二孢属、木霉属、镰刀菌属、角菌根菌属等11个种属,为进一步研究寒兰与其菌根真菌的共生关系和有益菌株的筛选奠定基础。
The mycorrhizal microstructure and the way of fungus infection of Cymbidium kanran roots were investigated and observed under optical microscope separately by freehand section method and aniline blue staining method. And, endophytic fungi from fresh myc- orrhizal roots of Cymbidiunl kanran were isolated and identified preliminarily by conventional isolation method. The results showed this species possessed the typical mycorrhizal structure of orchid plants. Mycorrhizal fungi invaded root cortical tissues by destroying the vela- men, and then formed peloton in parenchyma cells. 36 strains of mycorrhizal fungi were isolated and obtained, belonging to 11 genera, such as Eptographium, Rhizoctonia, Monilia, Diplodia, Trichoderma, Fusarium and Ceratorhiza, etc. It could provide reference for further research in the relationship between Cymbidium kanran plant growth and mycorrhizal fungus, and lay foundation of the screening out of beneficial strains.
作者
李国平
杨鹭生
李娟
LI Guoping;YANG Lusheng;LI Juan(School of Ecology and Resource Engineering, Wuyi University, Wuyishan, Fujian 35430)
出处
《武夷学院学报》
2018年第3期23-28,共6页
Journal of Wuyi University
基金
福建省科技创新平台建设计划项目(2017N2005)
福建省科技厅战略性新兴产业重点项目(2014N0030)
国家级大学生创新创业训练计划(201610397006)
关键词
菌根结构
菌根真菌
分离鉴定
寒兰
mycorrhizal microstructure
mycorrhizal fungus
isolation and idenfififion
Cymbidium kanran
