摘要
目的:探讨复方芩柏颗粒剂对溃疡性结肠炎大鼠p38MAPK通路的影响及可能的作用机制。方法:将80只SD大鼠随机分为正常组、模型组、阻断剂组和中药组,每组各20只。对除正常组外的其余组大鼠采用TNBS法制备溃疡性结肠炎大鼠模型,造模2周后各组分别予相应药物进行灌胃,采用免疫组化法检测结肠组织中p-p38MAPK、CK2的表达。结果:与正常组比较,模型组、阻断剂组、中药组中p-p38MAPK、CK2的表达水平明显升高,差异具有统计学意义(P>0.01);与模型组比较,阻断剂组、中药组中p-p38MAPK、CK2的表达水平明显降低,差异具有统计学意义(P>0.01);阻断剂组与中药组p-p38MAPK、CK2的表达水平差异无统计学意义(P>0.05)。结论:复方芩柏颗粒剂可能是通过抑制相关蛋白CK2的表达以弱化p38MAPK通路来发挥对溃疡性结肠炎的治疗作用。
Objective： To investigate the effect of compound Qinbo granules on the p38 mitogen-activated protein kinase（p38 MAPK） pathway in rats with ulcerative colitis and related mechanism of action. Methods： A total of 80 Sprague-Dawley rats were randomly divided into normal group,model group,antagonist group,and traditional Chinese medicine（TCM）group,with 20 rats in each group. All rats except those in the normal group were used to establish a rat model of ulcerative colitis using 2,4,6-trinitrobenzene sulfonic acid,and at 2 weeks after the model was established,the drugs were given by gavage. Immunohistochemistry was used to measure the expression of phosphorylated p38 MAPK（p-p38 MAPK） and CK2 in the colonic tissue. Results： The model group,the antagonist group,and the TCM group had significant increases in the expression of p-p38 MAPK and CK2,as compared with the normal group（P〈0. 01）. Compared with the model group,the antagonist group and the TCM group had significant reductions in the expression of p-p38 MAPK and CK2（P〈0. 01）. There were no significant differences in the expression of p-p38 MAPK and CK2 between the antagonist group and the TCM group（P〈0. 05）. Conclusion： Compound Qinbo granules exert a therapeutic effect on ulcerative colitis possibly by inhibiting the expression of CK2 and the p38 MAPK pathway.
作者
陆文洪
罗雯鹏
王真权
LU Wen - hong;LUO Wen - peng;WANG Zhen -quan(The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha 410005, Hunan, China)
出处
《湖南中医杂志》
2018年第4期136-137,共2页
Hunan Journal of Traditional Chinese Medicine
基金
湖南省中医药管理局资助项目(编号:201655)
关键词
溃疡性结肠炎
大鼠
复方芩柏颗粒剂
P-P38MAPK
CK2
实验研究
ulcerative colitis
rat
compound Qinbo granules
phosphorylated p38 mitogen - activated protein kinase
CK2
experimental study
