摘要
目的:分析比较不同产地山柰的挥发性成分,为后续药理活性研究奠定物质基础。方法:首次采用顶空固相微萃取-气相色谱质谱联用(HS-SPME-GC-MS)技术分别对广西、广东及云南山柰中挥发性化学成分进行提取和分析鉴定,并利用面积归一化法计算各成分的质量分数。结果:共鉴定出42种化学成分,主要为萜类、烃类、酯类和芳香族化合物。从广西山柰挥发性物质中分离出41个色谱峰,鉴定出38种化学成分,占挥发性成分总量的99.78%;从广东山柰挥发性物质中分离出37个色谱峰,鉴定出26种化学成分,占挥发性成分总量的80.49%;从云南山柰挥发性物质中分离出31个色谱峰,鉴定出24种化学成分,占挥发性成分总量的64.72%。结论:广西、广东及云南山柰的挥发性成分总体差异不大,3个产地的特征性成分均为甲氧基肉桂酸乙酯、肉桂酸乙酯及十五烷,但3个成分的相对含量大不相同,广西山柰中三者的相对含量分别为45.02%、17.18%、9.08%;广东山柰中三者的相对含量分别为41.08%、16.25%、8.04%;云南山柰中三者的相对含量分别为30.78%、15.66%、7.89%。其中甲氧基肉桂酸乙酯为山柰的主要活性成分之一,由此可推断广西、广东山柰质量优于云南山柰。本试验同时为山柰的道地性提供了佐证,并为山柰的进一步开发提供参考依据。
Objective： To lay the material foundation for the research of subsequent pharmacological activities by the analysis of volatile components in Kaempferia galanga Linn. from different origins. Methods： It was the first time that headspace solid-phase microextraction and gas chromatography mass spectrometry （HS-SPME/GC-MS） technique was used to extract and analyze the volatile chemical components in Kaempferia galanga Linn. from Guangxi, Guangdong and Yunnan, and the area normalization method was used to calculate the mass fraction of each component. Results： Totally 42 chemical constituents were identified, mainly terpenoids, hydrocarbons, esters and aromatic compounds. A total of 41 chromatographic peaks were isolated from the volatile substances in Guangxi and 38 chemical constituents were identified, which accounted for 99.78% of the total of the volatile components. Totally 37 chromatographic peaks were isolated from the volatile substances in Guangdong, and 26 chemical constituents were identified, which accounted for 80.49% of the total of the volatile components. A total of 31 chromatographic peaks were isolated from the volatile compounds of Yunnan, and 24 chemical constituents were identified, which accounted for 64.72% of the total of the volatile components. Conclusion： The volatile components in Kaempferia galanga Linn. from Guangxi, Guangdong and Yunnan show little difference, and the characteristic components of the three habitats are methoxy cinnamate ethyl cinnamate, ethyl cinnamate and pentadecane, however, the relative contents of the three characteristic components from the three areas are much different, which are 45.02% , 17.18% and 9.08% for Gnangxi, 41.08% , 16.25% and 8.04% for Guangdong, and 30.78% , 15.66% and 7.89% for Yunnan. One of the main active components in Kaempferia galanga Linn. is methoxy cinnamate ethyl cinnamate, which can he inferred that the quality of Kaempferia galanga Linn. from Guangxi and Guangdong is better than that from Yunnan. This experiment also provid
作者
黎强
余凡
Li Qiang;Yu Fan(Department of Pharmacy, Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology ,Wuhan 430030, Chin)
出处
《中国药师》
CAS
2018年第5期840-842,846,共4页
China Pharmacist
关键词
不同产地
山柰
顶空-固相微萃取
气相色谱-质谱联用
挥发性成分
Different habitats
Kaempferia galanga Linn.
Top space-solid phase microextraction
Gas chromatography-massspectrometry
Volatile component
