摘要
目的探究耳复康口服液对庆大霉素致豚鼠耳聋模型听觉耳动反射及瞬态诱发耳声发射(transient evoked otoacoustic emission,TEOAE)的影响。方法将豚鼠随机分为正常对照组,模型组,耳聋左慈丸组(2.7 g·kg^(-1)),高、中、低剂量耳复康口服液组(18,9,4.5 m L·kg^(-1))。除空白对照组每天肌肉注射生理盐水2 m L·kg^(-1),其余各组每天肌肉注射硫酸庆大霉素80 mg·kg^(-1),同时给予相应的药物进行灌胃14 d,分别于第7天、第14天给药后进行听觉耳动反射的测试,第15天时每只豚鼠进行TEOAE测试。结果对庆大霉素致豚鼠耳聋模型听觉耳动反射的影响:与模型组比,第7天耳复康口服液低剂量组可显著改善豚鼠听觉障碍(P<0.01);第14天耳复康口服液高、中、低剂量组均可显著改善豚鼠听觉障碍(P<0.01)。对庆大霉素致豚鼠耳聋模型TEOAE的影响:与模型组比,耳复康口服液各剂量组总反应能量显著降低(P<0.05或P<0.01);耳复康口服液各剂量组总相关度显著降低(P<0.01或P<0.05)。结论耳复康口服液均对庆大霉素致聋豚鼠的听力障碍有改善作用。
OBJECTIVE To investigate the effect of Erfukang oral liquid on hearing loss,hearing reflex and transient evoked otoacoustic emission（TEOAE）in guinea pigs with gentamicin induced hearing loss.METHODS The guinea pigs were randomly divided into control group,model group,Erlongzuociwan group（2.7 g·kg-1）,high,medium and low dose of Erfukang oral solution group（18,9,4.5 m L·kg-1）.In addition to the control group daily intramuscular injection of 2 m L·kg-1 saline,other groups were given daily intramuscular gentamicin sulfate 80 mg·kg-1,and given the corresponding drugs by gavage,injection（intragastric administration of drugs and injection of gentamicin was 14 d）,auditory ears reflected in seventh day and fourteenth day after administration were tested,fifteenth day guinea pigs in each TEOAE testing.RESULTS Effect of gentamicin induced auditory auditory reflex in the deafness model of guinea pig：on the seventh day,hearing ear reflected,the control group and the model group had significant difference（P〈0.01）,each dose group of Erfukang oral liquid dosage group compared with model group had significant difference（P〈0.01）.The influence of TEOAE model of gentamicin induced deafness in guinea pig ear：total reaction energy of each dose group of Erfukang oral liquid decreased significantly compared with control group（P〈0.05 or P〈0.01）;and correlation of each dose group of Erfukang oral liquid significantly decreased（P〈0.05 or P〈0.01）.CONCLUSION The treatment of Erfukang on which hearing loss caused by gentamicin in guinea pigs has improved effect.
作者
刘晓斌
郝少君
谢国旗
苏峰
管志江
张正臣
LIU Xiaobin;HAO Shaojun;XIE Guoqi;SU Feng;GUAN Zhijiang;ZHANG Zhengchen(The 371st Central Hospital of the Chinese People＇s Liberation Army, Xinxiang 453000, China)
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2018年第4期479-483,共5页
Chinese Journal of Modern Applied Pharmacy
基金
河南新乡重点科技攻关项目(ZG14014)
