摘要
为了筛选合适的生物标志物以指示水体镉(Cd^(2+))的污染程度,试验以背角无齿蚌(Anodonta woodiana)为材料,设置了2个Cd^(2+)质量浓度组(0.337,1.349 mg/L)和1个对照组,分别检测了镉暴露(0~28 d)和镉清除(28~56 d)过程中,背角无齿蚌鳃组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GPx)和过氧化氢酶(CAT)活性。结果表明,Cd^(2+)暴露后鳃组织SOD活性被抑制;GPx活性被诱导;CAT活性在低浓度组中表现为抑制,在高浓度组中表现为诱导。Cd^(2+)清除后,鳃组织中SOD活性依然被抑制,GPx活性依然被诱导,而CAT活性恢复到对照组水平。说明背角无齿蚌对镉污染具有一定的清除能力,抗氧化酶活性的变化能够反映生物体受污染物胁迫的程度,其可作为生物标志物用于指示水体镉污染状况。
To select the appropriate biomarkers to indicate the degree of cadmium contamination in water bodies, in this study, we used Anodonta woodiana as experimental animal and set two Cd^（2＋）treatment groups（0.337, 1.349 mg/L）and one control group. The activities of superoxide dismutase（SOD）, glutathione peroxidase（GPx）and catalase（CAT）in gill tissues were detected. The results showed that after the exposure of Cd the SOD activity was inhibited and GPx was induced in both high and low Cd^（2＋）group, and the CAT activity was inhibited in the low Cd^（2＋）group, but induced in the high Cd^（2＋）group. After the elimination of Cd, the activity of SOD remained to be inhibited, and the activity of GPx was still induced. The CAT activity was recovered to control group level. Thus, Anodonta woodiana had a certain ability to recover from Cd^（2＋）exposure. Also, the change of antioxidant enzyme activity could be used as a biomarkers to reflect the degree of organism being contaminated.
作者
井维鑫
刘娜
王兰
JING Weixin;LIU Na;WANG Lan(College of Life Science, Shanxi University, Taiyuan 030006, Chin)
出处
《山西农业科学》
2018年第5期752-755,共4页
Journal of Shanxi Agricultural Sciences
基金
山西省特色学科重点项目(2011-SXDX-SWX-003)
关键词
背角无齿蚌
抗氧化酶
氧化损伤
生物标志物
镉
Anodonta woodiana
antioxidant enzymes
oxidative damage
biomarkers
cadmium
