摘要
以亚硫酸盐(SPORL)和稀硫酸(DA)预处理蔗渣为底物,探讨两种预处理方法对蔗渣酶解的影响,并对酶解过程中蔗渣对纤维素酶的吸附特性进行探究,比较了两种底物的酶水解效率、成分、表面形貌、吸附等温线和吸附动力学等特征,结果显示:SPORL法预处理蔗渣的还原糖转化率(81.92%)明显高于DA法预处理蔗渣(60.89%)。与未处理蔗渣相比,DA与SPORL法预处理蔗渣表面变得粗糙,表面纤维被部分剥离,暴露出内部的纤维骨架。两种方法预处理蔗渣的纤维素和半纤维素含量也没有明显差异,但SPORL法预处理蔗渣中Klason木质素含量更低。X射线光电子能谱(XPS)图分析表明两种预处理蔗渣都对纤维素酶有明显吸附。吸附实验结果表明,SPORL法预处理蔗渣对纤维素酶最大吸附量为61.91 mg/g,低于DA法的82.69 mg/g,但SPORL法预处理蔗渣对纤维素酶的吸附亲和力及吸附强度均大于DA法。
The effects of two pretreatment methods,sulfite（ SPORL） pretreatment and dilute sulfuric acid（ DA） pretreatment,on the enzymatic hydrolysis of sugarcane bagasse（ SCB） substrates were investigated and the adsorption of cellulose on SCB during the hydrolysis was studied. The enzymatic hydrolysis efficiency,composition,surface morphology,adsorption isotherms and kinetics of two kinds of pretreated SCB were compared. The results showed that the enzymatic hydrolysis efficiency of SPORL-SCB（ 81.92%） was significantly higher than that of DA treated SCB（ 60.89 %）. Compared with the untreated SCB,the surface of SCB treated by SPORL and DA became rough,the surface fibres were partially stripped and the internal fibre skeleton was exposed. The holocellulose contents of these two treated SCB substrates were not significantly different,but the Klason lignin content of SPORL treated SCB was lower. The XPS images suggested that the treated SCB substrates both adsorbed cellulase. The adsorption isotherm and kinetics curves showed that the maximum adsorption of cellulase on SPORL treated SCB was 61.91 mg/g and lower than that on DA treated SCB（ 82.69 mg/g）,but the adsorption affinity and adsorption strength of SPORL treated SCB were higher than those of the DA treated SCB.
作者
董益帆
刘志佳
兰天晴
DONG Yifan;LIU Zhijia;LAN Tianqing(Food Safety Institute, Kunming University of Science and Technology, Kunming 650500, Chin)
出处
《林产化学与工业》
EI
CAS
CSCD
北大核心
2018年第2期75-79,共5页
Chemistry and Industry of Forest Products
基金
国家自然科学基金资助项目(21766014)
云南省科技计划面上项目(2014FB119)
云南省人才培养计划项目(KKSY201405054)
关键词
蔗渣
亚硫酸盐预处理
稀酸预处理
纤维素酶水解
纤维素酶吸附
sugarcane bagasse
sulfite pretreatment
dilute acid pretreatment
enzymatic hydrolysis
cellulase adsoqotion
