摘要
目的探讨姜黄素对大鼠脑缺血损伤后Janus蛋白酪氨酸激酶2(JAK2)/信号转导和转录激活子3(STAT3)信号通路的影响。方法将68只SD大鼠随机分为假手术组、脑缺血组、姜黄素组(尾静脉注射姜黄素40mg/kg)和对照组(注射等量生理盐水),每组17只,后3组建立大鼠局灶性脑缺血模型,观察各组大鼠神经行为学变化,ELISA检测大鼠脑组织TNF-α、白细胞介素(IL)-1β、IL-6表达,Western blot检测大鼠脑组织JAK2、磷酸化JAK2(p-JAK2)、STAT3、磷酸化STAT3(p-STAT3)、高迁移率族蛋白1(HMGB1)表达。结果与脑缺血组比较,姜黄素组大鼠神经行为学评分减低[(1.53±0.62)分vs(2.94±0.87)分,P<0.05];与脑缺血组比较,对照组TNF-α、IL-1β和IL-6无明显变化(P>0.05),姜黄素组大鼠TNF-α[(57.63±10.27)ng/L vs(99.35±8.97)ng/L]、IL-1β[(33.67±9.10)ng/L vs(58.43±7.22)ng/L]和IL-6[(31.97±6.91)ng/L vs(49.23±6.28)ng/L]表达降低(P<0.01),p-JAK2/JAK2、p-STAT3/STAT3相对含量及HMGB1表达降低(P<0.05,P<0.01)。结论姜黄素可保护大鼠缺血后脑损伤,其机制可能是通过抑制JAK2/STAT3信号通路,减少HMGB1表达,减轻炎性反应。
Objective To study the effect of curcumin on JAK2/STAT3 signaling pathway following ischemic cerebral injury in rats.Methods Sixty-eight SD rats were divided into sham operation group,cerebral ischemia group,curcumin treatment group and control group（17 in each group）.After a rat focal cerebral hemmorrahge model was established,the changes of neurological behaviors in rats were recorded,expressions of TNF-α,IL-1βand IL-6 were detected by ELISA and those of JAK2,p-JAK2,STAT3,p-STAT3 and HMGB1 were detected by Western blot.Results The neurological behavior score was lower in curcumin treatment group than in cerebral ischemia group（1.53±0.62 vs 2.94±0.87,P〈0.05）.No significant difference was found in expression levels of TNF-α,IL-1βand IL-6 between cerebral ischemia group and control group（P〈0.05）.The expression levels of TNF-α,IL-1βand IL-6 were lower in curcumin treatment group than in cerebral ischemia group（57.63±10.27 ng/L vs 99.35±8.97 ng/L,33.67±9.10 ng/L vs58.43±7.22 ng/L,31.97±6.91 ng/L vs 49.23±6.28 ng/L,P〈0.01）.The relative volume of p-JAK2/JAK2 and p-STAT3/STAT3 and the expression level of HMGB1 were lower in curcumin treatment group than in cerebral ischemia group（P〈0.05）.Conclusion Curcumin can protect rats against cerebral injury following ischemia by inhibiting the JAK2/STAT3 signaling pathway and downregulating the HMGB1 expression,and can thus alleviate inflammatory reactions.
作者
徐静
吴玉泉
许娟
戴毅
周春
费明峰
潘湛
Xu Jing;Wu Yuquan;Xu Juan;Dai Yi;Zhou Chun;Fei Mingfeng;Pan Zhan(Department of Geriatrics, Chinese P LA No. 117 Hospital, H angzhou 310013, Zhej iang Province, China)
出处
《中华老年心脑血管病杂志》
CAS
北大核心
2018年第5期534-537,共4页
Chinese Journal of Geriatric Heart,Brain and Vessel Diseases
基金
浙江省科技计划(2015C33179)
南京军区医药卫生科研基金(14MS147)
