摘要
目的观察A型肉毒毒素（botulinum toxin typeA，BTXA）对大鼠皮肤扩张速度及皮瓣即时回缩率的影响，探索药物辅助皮肤扩张的新方法。方法将18只SD大鼠随机分为BTX—A组和对照组，每组9只。BTX—A组和对照组分别于背部标记区域皮内注射BTX-A或生理盐水并埋置扩张器，术后定期注水至设定囊内压，记录扩张容量并定期测量标记区域面积，扩张4周后取材测量扩张皮肤即时回缩率，HE染色测量表皮、真皮、纤维包膜厚度，Masson染色观察包膜胶原沉积情况，α—SMA免疫组织化学染色评估包膜肌成纤维细胞含量。结果BTX—A组较对照组扩张容量大，标记区域面积大，皮肤即时回缩率低，纤维包膜薄，包膜胶原含量及α-SMA阳性的肌成纤维细胞少，差异有统计学意义（P〈0．05）。两组扩张皮肤表皮及真皮厚度差异无统计学意义（P〉0．05）。结论BTX—A能抑制纤维包膜形成从而促进皮肤扩张，同时降低扩张皮肤的即时回缩率，是一种较理想的辅助扩张的药物。
Objective To observe the effect of botulinum toxin type A （BTX-A） on the rate of skin expansion and the immediate retract rate of skin flaps in rats, and to explore new methods for drug-assisted skin expansion. Methods 18 Sprague-Dawley rats were randomly divided into two groups. After BTX-A or normal saline was injected intradermally into the back marking area, an ex- pander was implanted. The expanders were inflated periodically by injecting normal saline to reach the designated intraexpander pressure. The inflation volume was recorded and the area of marked region was measured regularly. After 4 weeks of expansion, the expanded tissues were harvested and the immediate retract rates were measured. The hematoxylin-eosin staining was performed to observe the thickness of epidermis, dermis and the fibrous capsule, while Masson staining for detection of fibrous capsule collagen. Immunohistochemical staining with a-smooth muscle actin for myofibroblasts was also performed. Results The mean inflation volume and area of marked region of BTX-A group were significantly greater than those of the control group. There were lower immediate retract rate, thinner fibrous capsule, less collagen content and fewer α-SMA positive myofibroblasts in the fibrous capsule of the BTX-A group with statistically significant differences （P 〈0.05）. There was no signigicant difference in the thicknesses of epidermis and dermis between the two groups （P〉0.05）. Conclusions BTX-A can inhibit the formation of fibrous capsule to promote skin expansion, while reducing the immediate retract rate. It possess a potential for assisting effectively skin exr^ansion.
作者
刘恒鑫
张曦
雷蕾
丁健科
崔江波
苏映军
马显杰
Liu Hengxin;Zhang Xi;Lei Lei;Ding Jianke;Cui Jiangbo;Su Yingjun;Ma Xianjie.(Department of Plastic Surgery, Xijing Hospital, the Fourth Military Medical University, Xi＇an 710032, China)
出处
《中华医学美学美容杂志》
2018年第2期125-129,共5页
Chinese Journal of Medical Aesthetics and Cosmetology
基金
国家自然科学基金资助项目（81272117,81671925）
