摘要
目的:筛选炎性痛和神经病理痛早期电针镇痛的优势频率并探讨其脊髓背角TRPV1活化的干预机制。方法:SD大鼠60只,随机分为空白组、炎性痛模型组、2Hz电针Ⅰ组、100Hz电针Ⅰ组、2/100Hz电针Ⅰ组;假模型组、神经病理痛模型组、2Hz电针Ⅱ组、100Hz电针Ⅱ组和2/100Hz电针Ⅱ组,每组6只。炎性痛模型选用弗氏完全佐剂(CFA)模型,神经病理痛模型选用坐骨神经分支选择性损伤(SNI)模型。取双侧足三里、昆仑进行电针干预。采用Von Frey丝检测各组大鼠造模后1d及电针治疗1、3d时患侧50%足底机械缩足反应阈值(PWTs);采用免疫印迹法测大鼠患侧SCDH TRPV1、p-TRPV1的表达。结果:100Hz电针升高CFA大鼠早期患侧PWTs的作用最显著(P<0.05);2Hz电针升高SNI大鼠早期患侧PWTs的效果最显著(P<0.01)。CFA和SNI模型组大鼠早期SCDH TRPV1、p-TRPV1蛋白表达显著升高(P<0.05);100Hz和2Hz电针分别降低了CFA和SNI大鼠早期SCDH TRPV1、p-TRPV1的蛋白表达(P<0.05)。结论:100Hz、2Hz电针分别为干预炎性痛和神经病理痛早期的优势频率电针;优势频率电针的镇痛效应可能通过调控炎性痛和神经病理痛大鼠早期SCDH TRPV1受体活化实现。
Objective： To select the optimal frequency of electroacupuncture（EA） analgesia in inflammatory pain and neuropathic pain and explore the interventional mechanism of TRPV1 activation in the spinal cord dorsal horn. Methods： Sixty SD rats were randomly divided into blank group, inflammatory pain model group, 2 Hz EA group Ⅰ, 100 Hz EA group Ⅱ, 2/100 Hz EA group Ⅰ; sham model group, neuropathic pain model group, 2 Hz EA group Ⅰ, 100 Hz EA group Ⅱand 2/100 Hz EA groupⅡ, n=6. Freund＇s complete adjuvant（CFA） model and spared nerve injury（SNI） model were established as inflammatory pain model and neuropathic pain model respectively. EA was administered at bilateral acupoints Zusanli（ST36） and Kunlun（BL60）. The affected side 50% paw withdrawal thresholds（PWTs） were detected by Von Frey hairs at time points of 1 day after modelestablishment, 1 day and 3 days after EA intervention in each group. The levels of TRPV1, p-TRPV1 in affected side SCDH were tested by Western blot. Results： Affected side PWTs of CFA rats in 100 Hz EA group Ⅰ were increased most obviously in EA groups（P〈0.05）, while affected side PWTs of SNI rats in 2 Hz EA group Ⅱ were increased most obviously in EA groups（P〈0.01）. The expression of TRPV1 and p-TRPV1 protein in SCDH were significantly increased in the early phase of both CFA and SNI rats（P〈0.05）; but 100 Hz EA and 2 Hz EA decreased the expression of SCDH TRPV1, p-TRPV1 level of CFA and SNI rats respectively（P〈0.05）. Conclusion： 100 Hz and 2 Hz are the optimal frequency of EA for early inflammatory pain and neuropathic pain. The analgesic effect of the optimal frequency EA may be accomplished by regulating the activation of SCDH TRPV1 receptor in early inflammatory pain and neuropathic pain models.
作者
项璇儿
杜俊英
房军帆
刘盈君
徐子童
王思思
孙海榉
方剑乔
XIANG Xuan-er;DU Jun-ying;FANG Jun-fan;LIU Ying-jun;XU Zi-tong;WANG Si-si;SUN Hai-ju;FANG Jian-qiao(Laboratory of Acupuncture Neurobiology, The Third Clinical Medical College of Zhejiang Chinese Medical University, Hangzhou 310053, China)
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2018年第5期1707-1712,共6页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金项目(No.81473772)
浙江省医药卫生重大科技计划(No.WKJ-ZJ-1419)
浙江省一流学科(中医学)资助(No.浙政办函[2016]6号)~~
关键词
炎性痛
神经病理痛
电针
优势频率
脊髓背角
TRPV1
p-TRPV1
Inflammatory pain
Neuropathic pain
Electroacupuncture
Optimal frequency
Spinal cord dorsal horn
TRPV 1
p-TRPV 1
