摘要
目的:研究益气活血风静胶囊对大鼠及家兔的血液流变学的影响。方法:采用SD大鼠及家兔分别灌胃给药14 d及10 d,检测SD大鼠及家兔血浆凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、凝血酶时间(TT)、纤维蛋白原(FIB)及血小板聚集率。另采用SD大鼠,大脑中动脉阻塞(MCAO)大鼠造模后灌胃给药5 d,检测MCAO大鼠PT、APTT、TT、FIB及脑组织含水量。结果:与空白组比较,益气活血风静胶囊高、中剂量组及阳性对照组,能显著延长大鼠血浆的PT、TT、APTT(P<0.05),并能显著降低FIB的含量(P<0.05或P<0.01),显著降低以二磷酸腺苷(ADP)诱导的大鼠血小板聚集率(P<0.05)。与空白组比较,益气活血风静胶囊高、中、低剂量组及阳性对照组能显著延长家兔血浆的PT、TT、APTT(P<0.05或P<0.01),并能显著降低FIB的含量(P<0.01),显著降低以ADP诱导的大鼠血小板聚集率(P<0.05或P<0.01)。MACO大鼠模型组给药后,与假手术组比较,模型组大鼠PT、APTT、TT明显缩短(P<0.05),FIB含量升高(P<0.05),脑组织含水量明显增加(P<0.01);与模型组比较,益气活血风静组和人参有效部位组能延长大鼠血浆PT、APTT、TT时间(P<0.05或P<0.01),降低纤维蛋白原的含量(P<0.01),显著降低模型大鼠脑组织含水量(P<0.05),牡丹皮有效部位组大鼠TT延长(P<0.05),降低纤维蛋白原的含量(P<0.01),显著降低模型大鼠脑组织含水量(P<0.05)。结论:益气活血风静胶囊具有显著的抗凝血以及抑制以ADP诱导的血小板聚集的作用,并对MCAO大鼠有较好的活血作用。
Objective： To investigate the effect of Yiqi Huoxue Fengjing Capsules on hemorheology of rats and rabbits. Methods： SD rats and rabbits were orally administrated for 14 days and 10 days respctively. The plasma prothrombin time （PT）, activated partial thromboplastin time （APTT）, thrombin time （TT）, fibrinogen （FIB） and platelet aggregation rate were measured in SD rats and rabbits. Furthermore, SD rats were used to establish middle cerebral artery occlusion （MCAO） rat model. The model was orally administrated for 5 days, then PT, APTT, TT,FIB and the water content of brain tissue in MCAO rats were measured. Results： Compared with the blank group, the Yiqi Huoxue Fengjing Capsules could significantly prolong the plasma PT, TT and APTT of rats （P 〈 0.05） of high and middle dose groups, and could remarkably reduce the content of Fib （P 〈 0.05 or P 〈 0.01） and the platelet aggregation induced by ADP of rats （P 〈 0.05）. Compared with the blank group, the Yiqi Huoxue Fengjing Capsules could significantly prolong the plasma PT, TT and APTT of rabbits （P 〈 0.05 or P 〈 0.01） of high, middle and low dose groups and the positive group, and could remarkably reduce the content of Fib of rabbits （P 〈 0.01） and the platelet aggregation induced by ADP on rats （P 〈 0.05 or P 〈 0.01）. Compared with sham-operated group, the PT, APTT and TT of MACO model group were significantly reduced （P 〈 0.05）, the content of FIB was significantly increased （P 〈 0.05）, and the water content of brain tissue was also significantly increased （P 〈 0.01）. Compared with MCAO model group of rats, the plasma PT, APTT and TT ofYiqi Huoxue Fengiing group and the effective components group of Genseng were significantly increased （P 〈 0.05 or P 〈 0.01）, the content of Fib was significantly reduced （P 〈 0.01） and the water content of brain tissue of MCAO model group was also significantly reduced （P 〈 0.05）. The TT of effective components group of Cortex
作者
李海剑
张婷婷
Li Haijian;Zhang Tingting(Henan Provincial Food and Drug Evaluation and Inspection Center, Zhengzhou 450000, China;Henan Provincial Institute of Food and Drug Control, Zhengzhou 450000, China)
出处
《中国药事》
CAS
2018年第4期522-528,共7页
Chinese Pharmaceutical Affairs
基金
河南省科技厅<基于新技术新方法的中药注射剂的安全性评价>项目(编号162102310067)
关键词
益气活血风静胶囊
血小板聚集
凝血指标
大脑中动脉阻塞大鼠模型
Yiqi Huoxue Fengjing capsules
platelet aggregation
blood coagulation indexes
middle cerebral artery occlusion rat model
