摘要
目的探讨冠心病患者血清微小核糖核酸(miR)-335表达和潜在转化生长因子结合蛋白2(LTBP-2)水平及其临床意义。方法选取2014年2月至2017年2月收治的96例冠心病患者为研究对象,按发病类型分为稳定型心绞痛(SAP)组34例,不稳定型心绞痛(UAP)组32例,急性心肌梗死(AMI)组30例;选取同期30例体检健康者作为对照组。采用实时荧光定量聚合酶链式反应(qRT-PCR)检测血清中miR-335表达水平,采用酶联免疫吸附试验(ELISA)检测血清中LTBP-2水平。结果随冠心病严重程度(按健康体检者、SAP、UAP、AMI之序)逐级加重,血清miR-335表达水平逐级降低(P<0.01),血清LTBP-2水平逐级升高(P<0.01),且各级患者间两两比较差异均有统计学意义(P均<0.05)。血清miR-335表达与Gensini评分呈明显负相关性(r=-0.532,P<0.05),血清LTBP-2表达与Gensini评分呈明显正相关性(r=0.796,P<0.01);冠心病患者血清中miR-335表达与LTBP-2水平呈明显负相关(r=-0.547,P<0.05)。结论 miR-335在冠心病患患者血清中呈明显低表达,LTBP-2水平显著增高,二者有望成为预测冠心病严重程度的参考指标。
Objective To explore the expression of serum microRNA（miR）-335 and the latent transforming growth factor（TGF）-β binding protein 2（LTBP-2） in patients with coronary heart disease（CHD） and the clinical significance.Methods A total of 96 patients with CHD from February 2014 to February 2017 including 34 stable angina pectoris（SAP） patients,32 unstable angina pectoris（UAP） patients and 30 acute myocardial infarction（AMI） patients were selected as research objects,and 30 healthy subjects of physical examination at the same time were selected as control group. Real time fluorescence quantitative polymerase chain reaction（qRT-PCR） was used to detect the expression level of serum miR-335. Enzyme-linked immunosorbent assay（ELISA） was used to detect the level of serum LTBP-2. Results With the severity of CHD（according to health check-up,SAP,UAP,AMI order） gradually increased,the expression level of serum miR-335 decreased gradually（P〈0. 01）,while the level of serum LTBP-2 increased（P〈0. 01）,and there were statistical differences between any two adjacent grades（all P〈0. 05）. There were a significant negative correlation between the expression level of serum miR-335 and the Gensini score（r =-0. 532,P〈0. 05） and a significant positive correlation between the level of serum LTBP-2 and the Gensini score（r = 0. 796,P〈0. 05） and a significant negative correlation between serum miR-335 expression level and serum LTBP-2 content（r =-0. 547,P〈0. 05）. Conclusion The miR-335 presents low expression in serum for CHD patients,and serum LTBP-2 level increases significantly,and both them are expected to be the reference indexes for predicting the severity of CHD disease.
作者
林能波
郑炜华
林永欢
LIN Neng-bo;ZHENG Wei-hua;LIN Yong-huan(Department of Cardiovascular Medicine, Dafeng Hospital of Chaoyang District, Shantou, Guangdong 515154, China)
出处
《中国临床研究》
CAS
2018年第3期317-320,325,共5页
Chinese Journal of Clinical Research
基金
广东省医学科学技术研究基金项目(B2015065)
