摘要
目的探讨微小RNA（miRNA，miR）-148b和miR-152在胆管癌中的表达及其生物学功能。
方法用实时定量聚合酶链反应（Real-time PCR）检测miR-148b和miR-152在胆管癌与正常胆管组织、胆管癌细胞株QBC939和良性胆管细胞株H-69中的表达；分别用Real-time PCR、噻唑蓝（MTT）检测法、克隆形成实验和流式细胞术检测miR-148b和miR-152的表达及其对细胞增殖、细胞周期和凋亡的影响。
结果与正常胆管组织比较，miR-148b和miR-152在胆管癌组织中分别下调（2.75±0.16）倍和（3.05±0.18）倍，差异均有统计学意义（P＝0.013、0.019）；与良性胆管细胞比较，miR-148b和miR-152在胆管癌细胞中分别下调（2.52±0.03）倍和（3.96±0.02）倍，差异均有统计学意义（P＝0.018、0.012）；miR-148b和miR-152过表达显著抑制胆管癌细胞的增殖速率，分别为（38.49±0.31）%和（52.69±1.01）%，同时也能显著抑制克隆形成率（P＝0.031、0.023），miR-148b和miR-152均可使细胞周期阻滞于S期（P＝0.027、0.017），并且miR-148b和miR-152过表达均可诱导细胞凋亡。
结论胆管癌组织中miR-148b和miR-152表达均下调，过表达miR-148b和miR-152后可抑制胆管癌细胞增殖及诱导胆管癌细胞凋亡。
ObjectiveTo investigate the microRNA （miRNA, miR）-148b and miR-152 expression and their biological functions in cholangiocarcinoma cells.
MethodsThe expression levels of miR-148b and miR-152 in cholangiocarcinoma simples and QBC939 cells were detected using real-time quantitative polymerase chain reaction （Real-time PCR）. After transfection with miR-148b/miR-152 mimics, cell proliferation, cell cycle and apoptosis were assessed using methyl thiazol tetrazolium （MTT） method, clone formation test and flow cytometry, respectively.
ResultsThe expression levels of miR-148b and miR-152 were significantly down-regulated [（2.75±0.16） folds and （3.05±0.18） folds] in cholangiocarcinoma samples as compared with normal samples （P=0.013, 0.019, respectively）, and [（2.52±0.03） folds and （3.96±0.02） folds] in QBC939 cells as compared with H-69 cells （P=0.018, 0.012, respectively）. Overexpression of miR-148b or miR-152 could significantly inhibit cell proliferation [（38.49±0.31）% and （52.69±1.01）%, respectively], and suppress cell clone formation （P=0.027, 0.017, respectively）, block cell cycle in the S phase and induce cell apoptosis.
ConclusionMiR-148b and miR-152 were down-regulated in cholangiocarcinoma cells, and overexpression of miR-148b and miR-152 could inhibit cell proliferation and induce cell apoptosis.
作者
曹坤
孙良权
龚勇军
张涛
李海洋
左石
Cao Kun;Sun Liangquan;Gong Yongjun;Zhang Tao;Li Haiyang;Zuo Shi(Department of General Surgery, Affiliated Hospital of Guizhou Medical University, Guiyang 550004, Chin)
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2018年第4期666-668,共3页
Chinese Journal of Experimental Surgery
基金
贵州省留学人员科技创新项目（2016-14）
