摘要
采用醇沉法提取了自30%~90%乙醇浓度下的分级多糖,分别标记为CSP-1、CSP-2、CSP-3、CSP-4、CSP-5、CSP-6、CSP-7,并以DPPH自由基清除率、还原力和铁离子螯合能力为模型分析了蝉花孢子粉分级多糖的体外抗氧化活性。实验结果表明:蝉花孢子粉分级多糖均显示了体外抗氧化活性,且抗氧化活性与多糖样品浓度间存在一定的正相关性。分级多糖CSP-4、CSP-5和CSP-6在浓度为(0.1~0.3)mg/mL时,自由基清除活性较好,CSP-6在浓度高于0.3 mg/mL时显著高于其他多糖样品(p<0.05),但与阳性对照Vc间存在明显差距;在还原能力上,CSP-6和CSP-5在浓度为(0.1~0.5)mg/mL时还原能力显著高于其他样品,但高于0.5 mg/mL时CSP-4的活性最为显著(p<0.05);在铁离子螯合能力方面,CSP-5与CSP-6在各实验浓度下活性均显著高于其他多糖样品(p<0.05)。综合评价3种抗氧化模型的实验结果,分级多糖CSP-5和CSP-6的体外抗氧化活性总体较为突出,值得对其进行进一步活性验证及纯化鉴定。
Rating polysaccharides were obtained from conidia powder of Cordyceps cicadae by fractional precipitation with different concentrations of ethanol（volume fraction 30% to 90%）, and respectively marked with CSP-1, CSP-2, CSP-3, CSP-4, CSP-5, CSP-6, CSP-7. The antioxidant activities in vitro of polysaccharides were investigated uisng 1,1-diphenyl-2-pierylhydrazyl（DPPH） radical scavenging activity assay, reducing power assay and chelating ability assay. The experiment results showed that all the rating polysaccharides had in vitro antioxidant activity and the effect was positively correlated with the concentration. Rating polysaccharides CSP-4, CSP-5 and CSP-6 had better scavenging activity on DPPH radical at the concentration of（0.1-0.3）mg/m L, when the concentration was above 0.3 mg/mL the scavenging activity of CSP-6 was higher than other samples obviously（p〈0.05）, although there was clear difference with positive control Vc. The reducing capacity of CSP-6 and CSP-5 was better than others at the concentration of（0.1-0.5）mg/mL and the capacity of CSP-4 was outstanding when the concentration was above 0.5 mg/mL（p〈0.05）. Chelating ability of ferric ion of rating polysaccharide CSP-5 and CSP-6 was higher than other polysaccharides significantly（p〈0.05）. The experiment results showed that the antioxidant activity in vitro of CSP-5 and CSP-6 was better, the two kind of polysaccharide were deserved to verify other activity and study the purification and identification process.
作者
邵颖
赵节昌
陈尚龙
巫永华
任格
孙颖
陈安徽
SHAO Ying;CHEN An-hui;ZHAO Jie-chang;CHEN Shang-long;WU Yong-hua;REN Ge;SUN Ying(College of Food （Biological） Engineering, Xuzhou University of Technology, Xuzhou 221111;Jiangsu Key Construction Laboratory of Food Resource Development and Quality Safe, Xuzhou University of Technology, Xuzhou 221111)
出处
《食品科技》
CAS
北大核心
2018年第4期200-204,共5页
Food Science and Technology
基金
江苏省科技计划项目(BN2016003,BE2017352)
关键词
蝉花
孢子粉
分级多糖
抗氧化
Cordyceps cicade
conidia powder
rating polysaccharides
antioxidant
