摘要
目的:制备负载光敏剂二氢卟吩e6(chlorin e6,Ce6)的金纳米星(gold nanostars,GNS),研究其对肺癌A549细胞的光动力作用效果。方法:GNS经巯基聚乙二醇(SH-PEG-NH2)修饰后与光敏剂Ce6振荡过夜,制备出具有光动力治疗效应的探针GNS-PEG@Ce6,检测其表征、形态和包封率,通过激光共聚焦显微镜比较A549细胞对探针GNS-PEG@Ce6与Ce6的吞噬作用。应用MTT法检测探针GNS-PEG@Ce6对A549细胞增殖的抑制效果,通过流式细胞仪检测探针GNS-PEG@Ce6与Ce6对A549细胞凋亡的影响。结果:探针GNS-PEG@Ce6的粒径为100 nm左右,具有良好的分散性和稳定性,Ce6的包封率为50%左右。探针GNS-PEG@Ce6以胞吞方式进入细胞,主要分布于细胞质内,且较Ce6能更有效地进入细胞。探针GNS-PEG@Ce6对A549细胞的增殖抑制作用强于Ce6(P<0.05),流式细胞仪检测证明了探针对细胞有极为明显的致凋亡效果。结论:以GNS作为载体能有效地增加肺癌A549细胞对Ce6的摄取,从而进一步增强Ce6对肺癌A549细胞的杀伤效果。
Objective： To prepare GNS（gold nanostars） loading photosensitizer chlorin e6（Ce6） and to investigate its photodynamic effects on lung cancer A549 cells. Methods： GNS was firstly modified by SH-PEG-NH2 and then mixed with Ce6 and shaken overnight to prepare GNS-PEG@Ce6, which had photodynamic therapy effects. The characterization, morphology and encapsulation rate were detected. The difference between the phagocytosis of Ce6 and GNS-PEG@Ce6 by A549 cells were observed with a Leical TCS SP8 confocal laser scanning microscope. MTT assay was used to examine the inhibitory effect of GNS-PEG@Ce6 on the proliferation of A549 cells while FCM was used to detect the effect of probe GNS-PEG@Ce6 on the apoptosis of A549 cells. Results： The particle size of the GNS-PEG@Ce6 was about 100 nm. The prepared GNS-PEG@Ce6 nanoparticles exhibited good dispersion and stability and the encapsulation rate of Ce6 was about 50%. GNS-PEG@Ce6 entered the cells by endocytosis and mainly distributed in the cytoplasm; compared with Ce6, GNS-PEG@Ce6 could enter the cells more effectively. The proliferation-suppression effect of GNS-PEG@Ce6 on A549 cells was significantly stronger than that of Ce6（P〈0.05）. The results of flow cytometry showed that the probe exhibited strong apoptotic effect on A549 cells. Conclusion： GNS, as the drug carrier, could effectively increase the Ce6 uptake efficacy in A549 cells,thus further enhancing the killing effects of Ce6 on lung cancer A549 cells.
作者
李晨露
夏芳芳
章阿敏
崔大祥
LI Chenlu;XIA Fangfang;ZHANG Amin;CUI Daxiang(School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou 325000, Zhejiang, China;Institute of Nano Biomedicine and Engineering , Shanghai Jiao Tong University, Shanghai 200240, China)
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
北大核心
2018年第4期394-400,共7页
Chinese Journal of Cancer Biotherapy
基金
国家重点研发计划资助项目(No.2017YFA0205301
No.2015CB931802)
上海市经信委资助项目(No.XC-ZXSJ-02-2016-05)~~
