摘要
目的探讨轻度胃肠炎伴良性婴幼儿惊厥(BICE)肠道微生物改变。方法选取2013年1月1日—2016年12月31日期间在瑞安市人民医院儿科诊治的BICE患儿45例为研究组,以2016年1月1日—2016年12月31日期间在瑞安市人民医院儿科诊治的急性轻度胃肠炎患儿45例为对照组,2组患者均行粪便轮状病毒检测和大肠杆菌、肠球菌、乳酸杆菌和双歧杆菌培养。抽空腹静脉血,测定肠黏膜屏障指标,包括二胺氧化酶(DAO)、一氧化氮(NO)和D-乳酸。结果研究组粪便轮状病毒阳性率(48.9%)显著高于对照组(26.7%,P<0.05)。研究组粪便大肠杆菌[(7.8±2.3)CFU/g]、肠球菌[(6.0±2.3)CFU/g]、乳酸杆菌[(4.9±1.7)CFU/g]和双歧杆菌[(5.1±1.5)CFU/g]分别低于对照组的(8.8±2.2)CFU/g、(7.3±2.8)CFU/g、(6.0±2.5)CFU/g和(5.9±1.9)CFU/g(均P<0.05)。研究组血清DAO[(9.84±4.17)U/L]、D-乳酸[(0.92±0.39)mmol/L]和NO[(126.8±48.1)μmol/L]分别高于对照组的(7.49±2.73)U/L、(0.73±0.25)mmol/L和(102.4±40.5)μmol/L(均P<0.05)。结论轮状病毒与BICE的发病关系密切,BICE患儿肠道内益生菌数量减少,肠黏膜屏障功能下降,补充益生菌或有助于BICE的防治。
Objective To explore the changes of intestinal microbiota in children with benign infantile convulsions asso- ciated with mild gastroenteritis （BICE）. Methods Forty-five cases of BICE diagnosed in our hospital between January, 2013 and December, 2016 were the study group. Forty-five cases of acute gastroenteritis diagnosed in our hospital be- tween January, 2013 and December, 2016 were the control group. Fecal rotavirus was detected and Escherichia coli, En- teroeoccus, Lactobacillus and Bifidobacterium were cultured in the two groups. Fasting venous blood was phlebotomized and intestinal mucosal barrier indexes including diamine oxidase （DAO） , nitric oxide （NO） and D-lactic acid were measured. Results The positive rate of fecal rotavirus in the study group （48.9%） was significantly higher than that in the control group （26.7 % ）, P 〈 0.05. The amounts of Escherichia coli [ （7.8 ± 2.3 ） CFU/g ], Enterococcus [ （ 6.0 ± 2.3 ） CFU/g ], Lactobacillus [ （4.9 ± 1.7 ） CFU/g ] and Bifidobacteria [ （5.1 ± 1.5 ） CFU/g ] in the study group were lower than the amounts of Escherichia coli [ （8.8 ± 2.2） CFU/g ], Enterocoecus [ （7.3 ± 2.8 ） ] CFU/g, Laetobacillus [ （6.0 ± 2.5 ） CFU/g] and Bifidobacteria [ （5.9 ± 1.9 ） CFU/g ] in the control group （ P 〈 0.05 ）. The serum level of DAO [ （9.84 ± 4.17） U/L ], D-lactic acid [ （0.92 ± 0. 39） mmol/L ] and NO [ （ 126.8 ,± 48.1 ） μmol/L ] in the study group were significantly higher than the serum level of DAO [ （7.49 ± 2.73） U/L], D-lactic acid [ （0.73 ± 0.25） mmol/Ll and NO[ （0.73 ±0.25）μmol/L] in the control group. Conclusion Rotavirus is closely associated with the pathogenesis of BICE. Probiotics in intestinal tract of BICE children reduce and intestinal mucosal barrier function de- crease, which means supplement of probiotics may help to prevent and treat BICE.
作者
卢乐声
徐智芳
白星
金伟敏
黄雨茅
金国信
吴昊澄
LU Le-sheng;XU Zhi-fang;BAI Xing(Department of Paediatrics, Jiaxing Maternity and Child health, Jiaxing, Zhejiang 314000, China)
出处
《中华全科医学》
2018年第5期802-804,共3页
Chinese Journal of General Practice
基金
2015年浙江省医药卫生平台计划(2015RCB008)
关键词
轻度胃肠炎伴良性婴幼儿惊厥
轮状病毒
微生态
肠黏膜屏障
Benign infantile convulsions associated with mild gastroenteritis
Rotavirus
Microecology
Intestinal muco-sal barrier
