摘要
以荒漠化土壤为试材,采用稀释平板和多相分类鉴定法,分离和鉴定了胞外多糖合成菌ZMN-3,并研究了该菌株的发酵液性能和保水效果,以期为荒漠化土壤的人工生物结皮固沙提供菌种资源和参考依据。结果表明:菌株ZMN-3属于马西利亚菌属(Massilia),其16SrRNA序列与Massilia plicata 76T的同源性最高(96.5%),结合系统进化和生理生化分析,判断ZMN-3应为马西利亚菌属的一个新种。菌株ZMN-3合成的胞外多糖包括2个组分,重均分子量Mw分别为1.91×10~4 kDa和947kDa;30℃发酵3d,ZMN-3发酵液粘度达到2 150mPa·s,呈粘稠的假塑性流体,喷洒于沙土表面后形成致密的生物结皮,具有明显的保水效果。
Desertified soil was used as experimental material.Polysaccharides synthetic strain ZMN-3 was isolated and identified by the method of dilution flat and polyphasic taxonomy.For the purpose of supply bacteria resources and reference for applying in artificial biological crust fix sand,properties of fermentation broth and water conservation of strain ZMN-3 were investigated.The results showed that strain ZMN-3 belonged to the genes of Massilia,exhibiting the highest 16 SrRNA sequence similarity with Massilia plicata 76 T（96.5% ）.On the basis of phylogenetics,physiological and biochemical analysis,strain ZMN-3 was considered to represent a novel species of the genus Massilia.The extracellular polysaccharide synthesized by ZMN-3 consisted of two components with different molecular weight distribution.The weight average molecular weight（Mw）were 1.91×10~4 kDa and 947 kDa,respectively.After incubation at 30 ℃for 3 days,the viscosity of ZMN-3 fermentation broth reached 2 150 mPa·s,as viscous pseudoplastic fluid.The microbial agent of ZMN-3 was sprayed on the surface of sand,compact bio-crust formed and the ability of water conservation of sand increased.
作者
任梦楠
金玉
李晓雁
李思琪
王艳梅
黄海东
REN Mengnan;JIN Yu;LI Xiaoyan;LI Siqi;WANG Yanmei;HUANG Haidong(College of Agronomy and Resources and Environment, Tianjin Agricultural University, Tianjin 300384;Department of Food Science and Biotechnology, Tianjin Agricultural University, Tianjin 300384)
出处
《北方园艺》
CAS
北大核心
2018年第8期23-28,共6页
Northern Horticulture
基金
国家自然科学基金面上资助项目(31571790)
天津农学院研究生培养质量提升资助项目(2017YJG009)
关键词
胞外多糖
马西利亚菌属
鉴定
保水效果
extracellular polysaccharide
Massilia
identification
water retention effect
